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Federica Blando Address for Correspondence


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Curriculum Vitae et Studiorum
Federica Blando

Address for Correspondence:

Istituto di Scienze delle Produzioni Alimentari (ISPA)

(Institute of Sciences of Food Production), CNR, Lecce Section

Via Prov.le Lecce Monteroni

73100 LECCE (Italy)

Tel.: +39.0832.422617

Mobile: +39.328.6211898

e-mail: federica.blando@ispa.cnr.it


Place of residence:

Via del Loto, 11



73020 Cavallino (Lecce, ITALY)
Date and place of Birth: 27th April 1962, Collesalvetti (LI), Italy
Nationality: Italian
Civil Status: married, with two children
Education:

  • 1987-1990 - University of Pisa - PhD in Horticultural Science (Curriculum: Propagation) obtained from the Department of Cultivation and Protection of Woody Species (Section: Fruit Tree Crops) in conjunction with the Institute of Mutagenesis and Differentiation – CNR (Italian National Research Council). Title of Thesis: “Characterisation of fruit tree suspension cultures capable of embryogenic differentiation”. Supervisors: Dr. Vittoria Nuti Ronchi and Prof. Stefano Morini.

  • 1981-1986 - University of Pisa in conjuction with Scuola Superiore di Studi Universitari e di Perfezionamento ‘S.Anna’ of Pisa: Bachelor of Science (Agricultural Science). Final Thesis in Genetics. : “Effect of Light and GA3 on the division of the first leaf in Triticum durum Desf.”. Supervisor: Prof. S. Baroncelli. Graduating marks: 110/110 with honours (full marks).

  • 1976-1981 – High School ‘Liceo Scientifico U. Dini’, Pisa. Final score: 60/60 (full marks)


Career/Employment:

  • 1997- present day. Permanent position as Researcher at the Institute of Sciences of Food Production, (I.S.P.A.), C.N.R. section of Lecce.

  • 1994-1997 - Temporary position as Researcher at the Institute of Research of Plant Food Biotechnology (I.R.B.A.), C.N.R., Lecce, Italy.

  • 1991-1993 - Post Doc Scholarship at the Fruit Tree Experimental Institute (Istituto Sperimentale per la Frutticoltura), in Rome. Title of research: “Somaclonal variation in strawberry cell cultures”. Supervisor: Dr. Carmine Damiano.



Stages and Mobility Grant:


  • June 2015-October 2015 – 18 weeks OECD Fellowship, which has been spent at the Australian Wine Research Institute (AWRI), Adelaide, Australia, under the supervision of Dr. Paul Smith and Dr. Keren Bindon.

  • September 2011 – 20 days visit to MTT- Jokioinen, Finland, ‘Bioavailability and efficacy of bioactive compounds’ course, tutor Dr. Anne Philanto.

  • From June 2007 to September 2007 – “Short Mobility Grant” from CNR to spend a period at the Food, Nutrition and Health Dept., University of British Columbia, Vancouver, Canada, in Dr. Kitts’ Laboratory.

  • 1992 – Short term (three months) visit to King’s College, London (U.K.), Biosphere Science Division, under the supervision of Prof. Peter Gahan. The activities were financed by the EU Programme on “Competitiveness in Agriculture and Management of Agriculture Resources 1989 – 1993”.


Scientific activity





  • During the PhD in Horticultural Science, the scientific activity was focused on the study of in vitro morphogenesis and differentiation of plant tissues. Particularly, the in vitro morphogenic process in several fruit tree crops, as apple and quince, was studied. The differentiation process of somatic embryogenesis was followed in a carrot model species.

Cytological techniques on in vitro and in vivo tissues were investigated and applied to different fruit tree crops.

  • During the fellowship at the Fruit Tree Experimental Institute in Rome, the scientific activity was focused on the setting up of protocols for the establishment of cell suspension cultures and regeneration in strawberry and kiwifruit. Furthermore, the selection of variant cell lines for salt tolerance in kiwifruit was performed exploiting somaclonal variation and in vitro screening.

  • At the Research Institute for Plant Food Biotechnology in Lecce, the scientific activity was focused on the study of the factor affecting quality in cherry fruits, in particular the softening of the fruits. Several enzymatic activities were followed during ripening. A major enzyme with ß-galactosidase / ß-glucosidase activities was purified as responsible for the increase of the softening of flesh fruit. Moreover, research on the induction of the regeneration of somatic tissue in several sweet cherry cultivars was carried out..

At the Institute of Sciences of Food Production (ISPA) in Lecce, the scientific activity has been focused on several goals:



  • characterization by HPLC of anthocyanin compounds extracted from red fruits (cherry, strawberry, grape, etc…) and wine, in particular typical wine from Apulia.

  • characterization of antioxidant activity of fruit extracts from Prunus species (sweet and sour cherry, mahaleb cherry) and other fruits (strawberry, grape, tomato) as well as wine.

  • exploitation of plant cell cultures as “bio-factories”, dealing with the in vitro production of natural antioxidants, particularly anthocyanins, from plant cell cultures of herbaceous and woody crop species. In vitro production of artemisinin (a natural compound with high antimalaric activity) from Artemisia annua cell cultures.

  • Studies on the in vitro regeneration processes.

Description of task and responsibility:


  • in the first period of the employment as researcher at IRBA, the main tasks were to perform experiments in order to carry out projects dealing with in vitro culture and regeneration of plant species, in particular fruit tree species, under the project leader (Dr. G. Zacheo) tutoring.

  • From years 1997 (with stable position as Researcher) the tasks were to carry out experiments and also to apply for funds, writing project proposal and discussing with national and international research groups.


Participation to National Projects and responsibility for one task:


  1. ‘Softening and Cherry’ (CNR project)

  2. ‘New biotechnological processes for in vitro production of natural antioxidants’ (CNR Project)

  3. ‘Artemisinin production in cell culture’ (Regional Apulia Project)

  4. ‘Innowine: a new approach for the characterization of autochthonous wines’ (Regional Apulia Project)

  5. ‘Chemical and biochemical characterization of Prunus mahaleb L. extract as a new food coloring pigment’ (Bilateral Project Italy-Israel financed by the Italian Ministry of Foreign Affairs)

  6. ‘High convenience fruits and vegetables: new technologies for the quality and new products (National Project PON 1_01435)


Responsability for the ‘In vitro Laboratory’:


  1. Tutoring of undergraduate students and PhD students for in vitro experiments.

  2. Supervision of the in vitro laboratory, staff and responsibility for dedicated instruments

Dr. Federica Blando has been a member, for years, of SOI (Italian Horticultural Society), of SIGA (Italian Society of Crop Plant Genetics) and of ISHS (International Society for Horticultural Sciences).



Publications:


  1. Mensuali-Sodi, A., G. Serra, C. Vitagliano, F. Blando (1990): “In vitro growth pattern of salt stressed cells of Lavandin”. Acta Hort (In Vitro Culture and Horticultural Breeding) (1°); 280: 459-462.




  1. Blando, F., L. Giorgetti, M. Tonelli, V. Nuti Ronchi (1992): “Cytological characterisation of cell suspension cultures of fruit trees”. Acta Hort (In Vitro Culture) 300: 377-380.




  1. Vitagliano, C., A. Mensuali-Sodi, F. Blando (1992): “Effect of NaCl on quince (Cydonia oblonga Mill.) tissue culture”. Acta Hort (In Vitro Culture) 300: 347-352.




  1. Blando, F., M. Tonelli, A. Frattarelli, C. Damiano (1993): “Establishment and morpho-cytological characterisation of cell suspension cultures in strawberry (Fragaria x ananassa Duch.) cv Pajaro”. Giornale Botanico Italiano, 127: 52-53.




  1. Blando, F., A. Niglio, A. Frattarelli, S. Speranza, C. Damiano (1993): “Cell suspension cultures in strawberry: growth characterisation and variability”. Acta Hort (In Vitro Culture and Horticultural Breeding) (2°) 336: 257-262.




  1. Blando, F., E. Caboni (1995): “Selezione in vitro di Actinidia per tolleranza a NaCl” (In vitro selection of Actinidia sp. for NaCl tolerance). Italus Hortus, 5-6: 32-36.




  1. Gerardi, C., A. Santino, F. Blando, G. Zacheo (1998): “Partial purification and characterisation of ß-D-Galactosidase from cherry (P. avium L., cv Lapins) fruits”. Journal of Biological Research, LXXIII Suppl. 9-10: 47-48.




  1. Gerardi, C., F. Blando, A. Santino, G. Zacheo (2001): “Purification and characterisation of a -glucosidase expressed in ripe sweet cherry (Prunus avium L.) fruit”. Plant Sci., 160 (5): 797-807.




  1. Blando F. (2002): “Propagazione in vitro di Prunus cerasus L.” (In vitro propagation of Prunus cerasus L.) Italus Hortus, 9 (3): 16-17.




  1. Gonzali S., Alpi A., Blando F., De Bellis L. (2002): “Arabidopsis (HXK1 e HXK2) and yeast (HXK2) hexokinases overexpressed in transgenic lines are characterized by different catalytic properties”. Plant Sci, 163: 943-954.




  1. Lucchesini M, Blando F., Mensuali Sodi A. (2003): “Coltivazione di specie officinali: dalle colture in vitro convenzionali ai protocolli alternativi di propagazione in vitro per la realizzazione di colture “microponiche”. (Medicinal plants: from conventional micropropagation to “microponic” culture) Italus Hortus, 10 (3): 166-168.




  1. Blando F., Gerardi C., Nicoletti I. (2004): “Sour cherry (Prunus cerasus L.) anthocyanins as ingredients for functional foods”. Journal of Biomed Biotech, 5: 253-258.




  1. Blando F., Scardino A.P., De Bellis L., Nicoletti I., Giovinazzo G. (2005): “Characterization of in vitro anthocyanin-producing sour cherry (Prunus cerasus L.) callus cultures”. Food Res Int, 38 (8-9): 937-942.




  1. Nutricati E., Miceli A., Blando F., De Bellis L. (2006) “Characterization of two Arabidopsis thaliana glutathione S-transferases”. Plant Cell Rep,25: 997-1005.




  1. Longo L., Scardino A., Vasapollo G., Blando F. (2007) “Anthocyanins from Eugenia myrtifolia Sims.”. Innov Food Sci Emerg, 8:329-332.




  1. Blando F., Chiriacò L., Gerardi C., Lucchesini M., Rampino P. (2007) “Sweet cherry (Prunus avium L.) ‘Giorgia’ adventitious regeneration from leaves of microplants. Europ. J. Hort. Sci., 72 (3):138-143.




  1. Blando F., Albrizio M., Marti L., Caretto S., Merendino A., Villanova L., Mita G. (2007) “Colture in vitro di Artemisia annua L. per la produzione del composto antimalarico artemisinina” (In vitro cultures of Artemisia annua L. for anti-malaric compound artemisinin production) In “Colture artificiali di piante medicinali” a cura di A. Paradossi, F. Tognoni, A. Mensuali, Aracne Ed, pp 224-232.




  1. Caretto S., Quarta A., Durante M., Nisi R., De Paolis A., Blando F. , Mita G. (2011) “Methyl jasmonate and miconazole differently affect arteminisin production and gene expression in Artemisia annua suspension cultures”. Plant Biol, 13 (1): 51-58.




  1. Ingrosso I., Bonsegna S., De Domenico S., Laddomada B., Blando F., Santino A., Giovinazzo G. (2011) “Overexpression of a grape stilbene synthase gene in tomato induces parthenocarpy and causes abnormal pollen development”. Plant Physiol Biochem, 49:1092-1099.




  1. Blando F., Spirito R., Gerardi C., Durante M., Nicoletti I. (2012) “Nutraceutical properties in organic strawberry from South Italy”. Acta Hort 926: 683-690.




  1. Gerardi C., Maltese F., Ali K., Blando F., Verpoorte R. (2012) “Metabolic characterization of Prunus cerasus L. and Prunus mahaleb L. fruits”. Acta Hort 940: 361-368.




  1. Gerardi C., Blando F., Santino A. (2012) “Purification and chemical characterization of a cell wall-associated -galactosidase from mature sweet cherry (Prunus avium L.) fruit”. Plant Physiol Biochem, 61: 123-130.




  1. Blando F., Onlu S., Colella G., Konczak I. (2013) “Plant regeneration from immature seeds of Eugenia myrtifolia Sims.”. In Vitro Cell Dev Biol-Plant. 49 (4): 388-395.




  1. Rizzello F., De Paolis A., Durante M., Blando F., Mita G., Caretto S. (2014) “Enhanced production of bioactive isoprenoid compounds from cell suspension cultures of Artemisia annua L. using β-cyclodextrins” Int. J. Mol. Sci., 15(10), 19092-19105.




  1. Albano C., Montefusco A., Blando F. (2014) “Caratterizzazione di polifenoli ed attività antiossidante (ORAC) in ciliegie di cultivar pugliesi”. Informatore Botanico Italiano, 328-330.




  1. Albano C., Negro C., Tommasi N., Gerardi C., Mita G., Miceli A., De Bellis L., Blando F. (2015) “Betalains, phenols and antioxidant capacity in cactus pear [Opuntia ficus-indica (L.) Mill.] fruits from Apulia (South Italy) genotypes”. Antioxidants, 4: 269-280. doi: 10.3390/antiox4020269.




  1. Gerardi C., Tommasi N., Albano C., Pinthus E., Rescio L., Blando F., Mita G. (2015) “Prunus mahaleb L. fruit extracts: a novel source for natural pigments”. European Food Research and Technology, 241 (5): 683-695.




  1. Blando F., Albano C., Yazheng L., Nicoletti I., Corradini D., Tommasi N., Gerardi C., Mita G., Kitts D.D. (2015) “Polyphenolic composition and antioxidant activity of the underutilized Prunus mahaleb L. fruit”. Journal of the Science of Food and Agriculture DOI:10.1002/jsfa.7381

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