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Mariana franco-ruiz

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    2006 to date: North Carolina State University. Department of Horticultural Science. PhD student, Research Assistant (full assistantship) Mapping origins of DNA replication in plants.

    2004-2005: North Carolina State University. Crop Science Department. Research scholar. Site-specific Recombination System.

    2002 to 2004: North Carolina State University. Botany Department. Research Scholar. Site-specific Recombination System.

  • Basic molecular biology techniques.

  • Generation of transgenic plants (Arabidopsis) with different reporter genes and target systems.

  • Crosses of transgenic lines (Arabidopsis and Nicothiana tobaccum)

  • PCR

  • Plant Tissue Culture (suspension cells, calli and calli transformation)

  • Plant DNA purification

  • Plasmid DNA purification

  • Plant RNA purification

  • Nuclei purification from callae

  • GUS Reporter gene detection

  • Photon Imaging (Luciferase assay)

  • GFP screening

  • Selection and maintenance of Arabidopsis transgenic lines.

  • Media preparation.

1999-2001 CINVESTAV (Center for Research and Advanced Studies, Plant Genetic Engineering Department) Irapuato, Mexico

Thesis work and Lab Technician

  • Bromatological Analysis of 3 different lines of Transgenic Potatoes Engineered for Virus Resistance. (Fat, Protein, Carbohydrates, Ashes and Fiber).

  • Protein profile of transgenic potato lines.

  • Plant Virus Maintenance (Inoculation and Purification).

  • Plant Virus Monitoring by ELISA and DAS-ELISA Procedures.

  • DNA bombardment (Gene gun) on pepper and tobacco plants for plant viruses inoculation.

  • Viral DNA Purifications.

  • Plant DNA Purifications.

  • RNA and DNA Virus interactions assays (In planta)

  • Scientific Photography Course.

  • Greenhouse and Growth Chambers plant assays.

  • Arabidopsis inoculation and maintenance.

  • Diverse Molecular Biology Techniques: Dot Blot, Southern Blot, Squash Blot, Gel electrophoresis (DNA and protein) etc.

1999 COFOCE (World Trade Promotion) Irapuato, Mexico.


  • Quality Control and Product Facilities Improvement for Product Export.

1994-1998 Instituto de Ciencias Agricolas (University of Guanajuato) Irapuato, Mexico.

Dairy Production Manager

  • Production.

  • Quality Control.

  • Sales.

1996-1997 GAB Inc. (Subsidiary of Doyle, Inc). Irapuato, Mexico.

Field Pest Control

  • Field Epidemiology.

  • Insect Control Spraying Program.

1995-1996 CESAVEG (USDA equivalent in Mexico) Irapuato,


Entomology Lab, Quality Control Officer

  • Biological Control Assays of parasitoid fungi against broccoli beetles.

  • Microbiological Quality Control.

  • Bioinsecticide production.


1994–1999 Universidad de Guanajuato (Instituto de Ciencias Agricolas) Irapuato, Guanajuato, Mexico.

  • Food Engineering (Agriculture)

  • Laureated Thesis "Proximal and Protein Analysis of Alpha, Norteña and Rosita transgenic potato lines transformed with PVX and PVY coat protein".

  • Patent # 37CFR §1.51(c)(1) (submitted)


Kumar, S., M. Franco, and G. C. Allen.  2006.  Gene targeting:  Development of Novel Systems for Genome Engineering in Plants. In: Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues. Chapter 8, pp. 84-98. J. A. Teixeira da Silva (ed). Global Science Books, London, UK

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