International union for the protection of new varieties of plants

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3. Method of Examination

3.1 Duration of Tests

The minimum duration of tests should normally be two independent growing cycles.

3.2 Testing Place

The tests should normally be conducted at one place. If any characteristics of the variety, which are relevant for the examination of DUS, cannot be observed at that place, the variety may be tested at an additional place.

3.3 Conditions for Conducting the Examination

The tests should be carried out under conditions ensuring satisfactory growth for the expression of the relevant characteristics of the variety and for the conduct of the examination.

3.3.1 Type of observation

The recommended method of observing the characteristic is indicated by the following key in the second column of the Table of Characteristics:
MG: single measurement of a group of plants or parts of plants

MS: measurement of a number of individual plants or parts of plants

VG: visual assessment by a single observation of a group of plants or parts of plants

VS: visual assessment by observation of individual plants or parts of plants

C: special test

3.4 Test Design

3.4.1 Each test should be designed to result in a total of at least 200 plants, which should be divided between two or more replicates.
3.4.2 The design of the tests should be such that plants or parts of plants may be removed for measurement or counting without prejudice to the observations which must be made up to the end of the growing cycle.

3.5 Number of Plants / Parts of Plants to be Examined

Unless otherwise indicated, all observations on single plants should be made on 30 plants or parts taken from each of 30 plants.

3.6 Additional Tests

Additional tests, for examining relevant characteristics, may be established.

4.Assessment of Distinctness, Uniformity and Stability

4.1 Distinctness

4.1.1 General Recommendations
It is of particular importance for users of these Test Guidelines to consult the General Introduction prior to making decisions regarding distinctness. However, the following points are provided for elaboration or emphasis in these Test Guidelines.
4.1.2 Consistent Differences
The minimum duration of tests recommended in Section 3.1 reflects, in general, the need to ensure that any differences in a characteristic are sufficiently consistent.

4.1.3 Clear Differences

Determining whether a difference between two varieties is clear depends on many factors, and should consider, in particular, the type of expression of the characteristic being examined, i.e. whether it is expressed in a qualitative, quantitative, or pseudo-qualitative manner. Therefore, it is important that users of these Test Guidelines are familiar with the recommendations contained in the General Introduction prior to making decisions regarding distinctness.

4.2 Uniformity

4.2.1 It is of particular importance for users of these Test Guidelines to consult the General Introduction prior to making decisions regarding uniformity. However, the following points are provided for elaboration or emphasis in these Test Guidelines:
4.2.2 For the assessment of uniformity, a population standard of 1% and an acceptance probability of at least 95% should be applied. In the case of a sample size of 200 plants, 5 off-types are allowed.

4.3 Stability

4.3.1 In practice, it is not usual to perform tests of stability that produce results as certain as those of the testing of distinctness and uniformity. However, experience has demonstrated that, for many types of variety, when a variety has been shown to be uniform, it can also be considered to be stable.
4.3.2 Where appropriate, or in cases of doubt, stability may be tested, either by growing a further generation, or by testing a new seed stock to ensure that it exhibits the same characteristics as those shown by the previous material supplied.

5. Grouping of Varieties and Organization of the Growing Trial

5.1 The selection of varieties of common knowledge to be grown in the trial with the candidate varieties and the way in which these varieties are divided into groups to facilitate the assessment of distinctness is aided by the use of grouping characteristics.
5.2 Grouping characteristics are those in which the documented states of expression, even where produced at different locations, can be used, either individually or in combination with other such characteristics: (a) to select varieties of common knowledge that can be excluded from the growing trial used for examination of distinctness; and (b) to organize the growing trial so that similar varieties are grouped together.
5.3 The following have been agreed as useful grouping characteristics:
(a) Grain: bitter principle (characteristic 1)

(b) Flower: color of wings (characteristic 9)

(c) Plant: growth type (characteristic 11)
5.4 Guidance for the use of grouping characteristics, in the process of examining distinctness, is provided through the General Introduction.

6. Introduction to the Table of Characteristics

6.1 Categories of Characteristics

6.1.1 Standard Test Guidelines Characteristics

Standard Test Guidelines characteristics are those which are approved by UPOV for examination of DUS and from which members of the Union can select those suitable for their particular circumstances.

6.1.2 Asterisked Characteristics

Asterisked characteristics (denoted by *) are those included in the Test Guidelines which are important for the international harmonization of variety descriptions and should always be examined for DUS and included in the variety description by all members of the Union, except when the state of expression of a preceding characteristic or regional environmental conditions render this inappropriate.

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