(Times New Roman 12, bold, center. Give affiliation and complete address for all authors) (E-mail: Alexandre.firstname.lastname@example.org)
(Times New Roman 12, center, Give the E-mail address of the presenting author only) Perennial ryegrass is of great economic importance to the leisure industries and Irish agriculture, accounting for 90% of the agricultural land. One of the objectives of breeding programs is to produce improved cultivars with a response for an increasing range of specific uses/situations. A stepwise approach has been conducted to investigate the transcriptional basis of phenotypic and metabolic plasticity to drought for a set of perennial ryegrass accessions. An ecotype, noted for drought tolerance, was obtained from the USDA GRIN database and was used together with a commercial variety to investigate metabolomic and transcriptional profiles under a PEG induced drought stress. Hydroponics based experiments showed the drought tolerant ecotype to increase root biomass in response to a drought stress and maintain a higher relative water content (RWC) relative to the variety. The use of suppression subtractive hybridisation combined with real-time RT-PCR has enabled the identification of transcripts up-regulated in the drought tolerant ecotype. The use of GC-TOF-MS has facilitated the identification of changes in the metabolome of perennial ryegrass under drought stress in both root and leaf tissue.
(The abstract should state briefly the purpose of the research, the method to achieve the results, the principal results and major conclusions. Abstract max 300 words. No paragraphs. Justified. Line spacing – single. A single line interval between the title, the authors list, email address and the text of the abstract)