Table S6 Cloning and identification of
|
|  Yüklə 89.5 Kb.
|
| Table S6 Cloning and identification of LcActin
S 6-1Total RNA isolated from Litchi chinensis Sonn. cv. Nuomici 
QLcactinF : ATGGCCGATGCTGAGGACATTC（5’→3’） QLcactinR : TCAGAAGCACTTCCTGTGGACAATG（5’→3’） Full-Length：1134bp
Lane 1：DL2000 Marker；Lane 2：PCR segment S6-4 DNA sequencing (Beijing Genomics Institute for sequencing) Result：(Full-Length 1134bp, Red letters represent binding domains with the primers) atggccgatgctgaggacattcagcctctcgtttgcgacaatggaactgggatggtgaaggctggatttgctggggatgatgcccccagggcggtgtttcccagtattgttggtcggccccgacacactggtgtgatggttggtatgggtcagaaggatgcctatgttggtgatgaggcccaatcgaaaagaggtattcttaccttgaaatacccgattgaacatggtattgtcagcaactgggatgatatggaaaagatctggcatcacactttctacaatgagcttcgtgttgctccagaagagcacccggtgcttcttactgaggctcctttaaatcccaaggccaacagagagaagatgacccagattatgtttgaaacttttaatgtgcctgccatgtatgttgccatccaggccgtcctctctctttacgccagtggtcgcacaactggtatcgtgctggattctggtgatggtgtgtctcatactgtgccaatctatgaaggatatgcccttcctcatgctattctccgtttggacctggctggtcgtgatctcaccgatgcattgatgaagattctgaccgagagaggttacatgtttaccaccactgccgaacgggaaattgtccgtgacatgaaagagaagcttgcctatgtcgcgctagactatgagcaggaacttgagactgccaagagcagctcctctgtggagaagaactatgagttacctgatggtcaggtcatcactattggagctgagagattccgttgcccagaagtcctcttccagccatctctcatcggaatggaagctgctggtatccatgagactacctacaactccatcatgaagtgtgatgtggatatcaggaaggatctctatggtaacattgtgctcagtggtggttctactatgttccctggtattgccgaccgtatgagcaaggaaatcactgcccttgccccaagcagcatgaagatcaaggttgtggctccacctgagagaaaatacagtgtctggattggaggatcaatcctcgcatctctcagcaccttccaacagatgtggatttcaaaggatgagtacgacgagtctggtccatccattgtccacaggaagtgcttctga S6-5 Alignment and phylogenetic analysis
This gene sequence has been submitted to the NCBI GenBank and the accession number is HQ615689. |
