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Supplementary information Hybrid hydrogels containing vertically aligned carbon nanotubes with anisotropic electrical conductivity for muscle myofiber fabrication


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Supplementary information
Hybrid hydrogels containing vertically aligned carbon nanotubes with anisotropic electrical conductivity for muscle myofiber fabrication
Samad Ahadian1,, Javier Ramón-Azcón1,, Mehdi Estili2, Xiaobin Liang1, Serge Ostrovidov1, Hitoshi Shiku3, Murugan Ramalingam1,4,5, Ken Nakajima1, Yoshio Sakka6, Hojae Bae7, Tomokazu Matsue1,3,*, Ali Khademhosseini1,8,9,10,11,*
1WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8577, Japan

2International Center for Young Scientists (ICYS), National Institute for Materials Science (NIMS), Tsukuba 305-0047, Japan

3Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan

4Centre for Stem Cell Research, A unit of the Institute for Stem Cell Biology and Regenerative Medicine, ChristianMedical College Campus, Vellore 632002, India

5Institut National de la Santé Et de la Recherche Médicale U977, Faculté de Chirurgie Dentaire, Université de Strasbourg, Strasbourg 67085, France

6Materials Processing Unit, National Institute for Materials Science (NIMS), Tsukuba 305-0047, Japan

7College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, Hwayang-dong, Kwangjin-gu, Seoul 143-701, Republic of Korea

8Department of Medicine, Center for Biomedical Engineering, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA

9Harvard–MIT Division of Health Sciences and Technology, Massachusetts Institute of

Technology, Cambridge, Massachusetts 02139, USA



10Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts 02115, USA

11Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 130-701, Republic of Korea



Figure S1. Anisotropic conductivity of pristine GelMA and hybrid GelMA-horizontally aligned CNT gels containing different concentrations of CNTs. (A) Schematic of the systems used for horizontal and vertical measurements of electrical conductivity for hybrid GelMA-horizontally aligned CNT hydrogels. (B) Impedance measurements of pristine GelMA and hybrid gels loaded with 0.5 and 1 mg/mL CNTs. The perturbation amplitude was 25 mV. (C) I-V curves for pristine GelMA and GelMA hydrogels containing aligned and random CNTs.



*Corresponding authors: Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan. Fax: +81 22 795 6167 (T. Matsue). Center for Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA. Fax: +1 617 768 8477 (A. Khademhosseini).

E-mail addresses: matsue@bioinfo.che.tohoku.ac.jp (T. Matsue) and alik@rics.bwh.harvard.edu (A. Khademhosseini).



These authors contributed equally to this work.





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