Ratios sh 10: 90; sha 0: 100; shb 100: 0; shc 50: 50; shd 25: 75; she 90: 10; shf 10: 0 and shg 0: 90

Yüklə 150 Kb.

tarix	24.06.2016
ölçüsü	150 Kb.

Costa et al.

Legends
Supplementary Fig. 1. Fresh weight (A, B) growth curves of lovage hairy roots grown in darkness (A, C) and under 16h light/8h dark photoperiod conditions (B, D) in SH (

), SHa (

), SHb (

), SHc (

), SHd (

), SHe (

), SHf (

) and SHg (

) liquid media. NH₄⁺:NO₃^- ratios SH 10:90; SHa 0:100; SHb 100:0; SHc 50:50; SHd 25:75; SHe 90:10; SHf 10:0 and SHg 0:90.

Supplementary Fig. 2. Examples of the components present in the Levisticum officinale hairy root volatiles and their relation to the main biosynthetic pathways.

Supplementary Fig. 3. Dendrogram obtained by cluster analysis of the percentage composition of volatiles from L. officinale hairy roots, maintained in media with different ammonium:nitrate ratios (SH 10:90; SHa 0:100; SHb 100:0; SHc 50:50; SHd 25:75; SHe 90:10; SHf 10:0 and SHg 0:90), in darkness (D) and under 16h light/8h dark photoperiod (P) conditions, based on correlation and using the unweighted pair-group method with arithmetic average (UPGMA). The measure of the goodness of fit between the cophenetic value obtained from the dendrogram and the correlation matrix showed a good fit with a cophenetic correlation value of 0.82.

Supplementary Fig. 4. Mass spectrum of the unidentified compound (RI=1835) found in the mixtures of volatiles isolated from lovage hairy roots.

A

B

Supplementary Fig. 1.

Supplementary Fig. 2.

Supplementary Fig. 3.

Supplementary Fig. 4.

Supplementary Table 1. Percentage composition of the minor components of the mixtures of volatiles isolated from lovage hairy root cultures, i.e., those that appeared in relative amounts <5% at least once in the volatiles of cultures grown in media with different ammonium:nitrate ratios, maintained in darkness or photoperiod conditions. NH₄⁺:NO₃^- ratios SH 10:90; SHa 0:100; SHb 100:0; SHc 50:50; SHd 25:75; SHe 90:10; SHf 10:0 and SHg 0:90.

		Culture medium
		Darkness									16h light/8h dark
Components	RI	SH	SHa	SHb	SHc	SHd	SHe	SHf	SHg		SH	SHa	SHb	SHc	SHd	SHe	SHf	SHg
trans-Hex-2-enal	866	t			t	t
cis-Hex-3-en-1-ol	868	t			t	t
n-Hexanol	882	t	t		1.1	t	t	1.9	t		0.1	t	t	3.0	t	t		4.1
2-Heptanone	886	t	t		t	3.7	t	t	t		0.5	t		t	t	t		t
Benzaldehyde	927	0.2	t		t	t		t			0.3	t		t	t	t		t
-Pinene	930	0.2			t	t
n-Heptanol	952	t	t		0.1	0.1	t	t	t		0.2			2.1		t		t
Oct-1-en-3-ol	961	t	t		t	0.1	0.1	t
-Pinene	963	0.2			0.3	t					0.2					t
2-Octanone	967	t	t		0.8	0.4	0.8	t	t		0.8		t	0.2	0.2	1.0		t
Benzene acetaldehyde	1002	0.1	t		0.2	0.1	t	t			0.4	t	t	3.3	t	t		t
p-Cymene	1003	t			t	t
Cineole	1005	1.2			t	t
-Phellandrene	1005	0.2	t		0.2	t	t				0.1	t				t		t
Limonene	1009	t			t	0.9	0.2	t			0.1				t	1.0
2-Nonanone	1058	0.1	0.1	t	t	1.0		t	t		0.5	0.8	t	0.2	t	t		4.1
n-Nonanal	1073	0.6	0.1	t	0.9	0.9	1.0		t		1.2	1.5	t		2.2	2.9		2.0
trans-Vertocitral	1077	0.3	t		0.9	1.3	1.1	t	t		1.9	t		t		1.9		t
Pentylcyclohexadiene	1125	0.1			t	0.1
Octanoic acid	1159	t	t		0.2	0.4	0.2				0.3	2.3			t	t		t
trans,trans-Nonadi-2,4-enal	1184	t			0.2	0.4					0.9	t		t		t
n-Dodecane	1200	t		t	0.4	0.4					0.3	t		2.3		t		t
2-Undecanone	1271	0.1			t	0.4	0.5				0.6	1.5			t	1.9		t
trans,trans-Decadi-2,4-enal	1286	0.1	t		0.5	1.7	0.7	t	t		0.4	t				t		t
Elemene	1388	0.1			t	t
-Elemene*	1441	1.7	1.8		1.3	t	1.3				1.2	1.5				t
trans--Farnesene	1456	0.7	0.1		t	t	2.7		t		0.3	0.8		t
Germacrene-D	1474	0.6			t
trans-Tridec-2-enal	1487	0.2			t	t	t				0.6
14-Hydroxy--muurolene*	1494	0.4			0.3	t	t				0.3		t			t
Germacrene-B	1533	1.7	0.7		1.3	1.2	1.0		t		0.6	1.5						t
(E)-Ligustilide	1669	0.5			t	t

RI: Retention index relative to C₈-C₂₂ n-alkanes on the DB-1 column; t: traces (<0.05%). * Identification based on mass spectra only.

Verilənlər bazası müəlliflik hüququ ilə müdafiə olunur ©atelim.com 2016
rəhbərliyinə müraciət