Pattari lohitha department of pharmacology h. S. K. College of pharmacy

NAME OF THE CANDIDATE AND ADDRESS

DEPARTMENT OF PHARMACOLOGY

H.S.K.COLLEGE OF PHARMACY

B.V.V.S.CAMPUS

BAGALKOT-587101. KARNATAKA.

NAME OF THE INSTITUTION

H.S.K.COLLEGE OF PHARMACY

B.V.V.S.CAMPUS

BAGALKOT-587101. KARNATAKA.

MASTER OF PHARMACY IN PHARMACOLOGY

DATE OF ADMISSION TO COURSE

JUNE 2007

TITLE OF THE TOPIC:

BRIEF RESUME OF THE INTENDED WORK

1. 
   Need for the study:
   

Stress plays an important role in the development of various affective disorders and responsible for various disease like hypertension, diabetes, cardiovascular disorders and effect on reproductive functions¹. The reproductive functions changes by life style related factors and with stress involve reduced fertility then impaired hormonal secretions, reduced viability of offspring and related sexual behavior². And reduced sperm concentration semen quality³. During the past decades several reports have suggested a time and stress related decline in sperm quality⁴. A meta analysis of 63 studies mainly from USA and from Europe indicated almost 50% reduction from 113x10⁶/ml to 65x10⁶ /ml in the mean semen concentration, during the period 1940 to 1950⁵.

It has been shown that there is a close co- relation between the sperm concentration and fertility potential of male⁶. In the western society as many as 7.5% of all male factors in at least 25% of such cases. Thus, failure of reproductive organ is encountered by a significant proportion of man and represents significant health burden since reproduction is one of the most important aspect of life of majority of individuals. The present available drugs in the market for the treatment of erectile dysfunction, sildanafil citrate and tadafil. These drugs have severe cardiovascular hypotension, hypersensitivity, myalgia, abnormalities and prolonged causes of infertility⁷. Only few drugs in the market, which overcomes from the impotency, infertility in male without any side effects.

Ancient people of India in Indian system of medicine has quoted several herbal products to overcome this conditions, on this basis and literature available from traditional claim of Listea glutinosa for its applicability as a aphrodisiac and in infertility, but literature review suggests that no scientific work has been carried out till date. Hence this plant has been selected for the studies. So these research studies helps in overcoming of impotency and reproductive associated problems with respect to environmental changes

Title of plant : Litsea glutinosa

Family : Lauraceae

Synonyms : Maidalatki, vasa. Garbijaur, kurkuchita

Parts used : Bark

Chemical constituents: Actinodaphnine, B- sitosterol, boldine, norboldine, laurotetanine,N- Methyllaurotetanine, N- methylactidaphnine, quercetine, kaempferol-3- glucoside, quercetin, kaempferol -7- glucoside, pelargonidine -5- glucoside, aminoacids, mono and sesquiterpenes, sebiferine, litsiferine^{8, 9.}

Medicinal action and uses: According to the treatise on Indian medicinal plants oil from berries is used in rheumatism. Bark is demulcent, aphrodisiac and anodyne, used in the treatment of diarrhea and dysentery sprains rheumatic gouty joints, acts as local antidote to bites of venomouse animals. Leaves are mucilaginous, employed in poultices for wounds and bruises. Decoction of roots used as an emmenagogue. Oil from berries is having anti-rheumatic activity^{8, 9.}

Pharmacological investigation shows this plant has shown scientifically antibacterial ¹⁰, cytotoxic activity¹¹.

The botanist in ayurvedic college in our college campus was authenticated bark was collected and extracted using non-polar and polar solvents. The extract used for the present study, with the following objectives:

1. 
   In vivo evaluation of aphrodisiac activity of Litsea glutinosa extract
   
2. 
   In vitro study on effect of extract on various parameters of semen
   
3. 
   To observe fertility activity on sterile male rats.
   

7.1 Source of data: For the evaluation of above said activity animals will be subdivided in to following

groups containing six animal in each group

1. 
   Effect of vehicle on sexual behavior.
   
2. 
   Effect of low dose of extract on sexual behavior.
   
3. 
   Effect of moderate dose of extract on sexual behavior.
   
4. 
   Effect of high dose of extract on sexual behavior.
   
5. 
   Effect vehicle normal animal group
   
6. 
   Effect of stress on sexual activity
   
7. 
   Effect of stress + standard drug on sexual activity
   
8. 
   Effect of low dose of extract + stress on sexual activity.
   
9. 
   Effect of moderate dose of extract + stress on sexual activity.
   
10. 
    Effect of high dose of extract + stress on sexual activity
    

Plant material : Litsea glutinosa

Animals : Male Wister albino rats

Chemicals : All chemicals to be used in the present study are AR grade

Instruments : Spectrophotometer, triangular microscope, refrigeration centrifuge,

haemocytometer, animal balance etc.

7.3 Methods:

Preparation of Litsea glutinosa extract: The bark authenticated and collected in the ideal collections in the air dried under the shade and powdered to be a fine powder of uniform size by passing through the sieve number 40. The powder collected will be extracted with Petroleum ether, chloroform, ethanol etc. the percent yield will be calculated and dose will be selected based on acute toxicity study¹². It will be administered after reconstitution in normal saline

of rats and also sacrifice to study the sperm characters. This will be done in accordance procedure reported in literature.

7.5 Has ethical clearance been obtained from your institution for performing various tests on animals?

Yes, the study is cleared from institutional animals ethics committee and the copy is enclosed with the protocol.

1. Schellenbaum GD, Rea TD, Smith NL. Menal stress induced ischemia and all cause mortality in

patients with coronary artery disease, circulation 2002:106:183-84.

2. Richtoff J, Rylander L, Hagmar L, Malm J, Giwercman A. Higher sperm counts in compared with

Denmark. Hum Report.2002; 17:2468-2473.

3. Colborn T, Clement C. Chemically induced alteration in sexual and functional development: the

Wildlife/human connection. Advances in modern Environmental toxicology ,1^sted. Princeton

scientific publication. Co 1992.

4. Nelson CMK, Bunge RG. Semen analysis: evidence for changing parameters of male fertility

potential. Fertil Sterile 1974; 25:503-507.

5. Auger J, Kunstmann J, Czyglik E, Jouannet P. Decline in semen quality among fertile men in Paris during the past 20 years. New England J med 1995; 332:281-285.

6. Bonde JPE, Ernst E, Jensen TK, Hjollund NHI, Kolstad H Henriksen TB. Relation between semen

quality and fertility: a population – based study of 430 first pregnancy planners. Lancet 1998;

352:1172-1177.

7. Malik S. Drugs for erectile dysfunction. Indian Drug Review 2004; 10:316-319.

8. Asima chatterjee and satyesh Chandra prakashi, The Treatise on Indian medicinal plants, by

publications and information directorate, New Dehli. 1999; 1: 06-107,

9. CSIR, The useful plants of India, New Dehli 1992, 334

10. Mandal SC, Kumar CK, Majumder R, Maity BC. Anti bacterial activity of Litsea glutinosa bark.

Fitoterapia.2000 Aug 71(4): 439-41.

11. Cytotoxic activity of Litsea glutinosa, doi.wiley.com/10,1002/hlca, 2005

12. OECD guidelines; acute oral toxicity; up and down procedure; September 1998;1-8.

13. Ratnasoorias WD, Dharmasiri MG. Effect of Terminalia catappa seeds on sexual behavior and

fertility of male rats. Asian J androl 2000; 2: 213-219.

14. Ghosewar MG, Ahemed NR, Ahmed M Aladakatti RH. Azardirachta indica adversely affects

Sperm parameters and fructose levels in vas deference fluid of albino rats. Bas Physiol

Pharmacol 2003; 14:387-395.

15. Johnson E, Kamilaris T, Chrousos G, Gold P. Mechanism of stress: a dynamic overview of

hormonal and behavioral homeostasis. Neurosci Bio Behav Rev 1992; 16:115-130.

.

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The immobilization causes the stress and it reduces the sperm count , motility and latency period was decreased markedly are the important characterers of impotency and our main objective was to find out traditional claim of Litsea glutinosa has improves fertility in male rats .

Prof. I.S.MUCHANDI

Department of Pharmacology

Prof. I.S.MUCHANDI

H.S.K.College of Pharmacy,

B.V.V.S. Campus, Bagalkot-587101.

The above mentioned information is correct and I recommended the same for approval.

Prof. I.S.MUCHANDI

H.S.K.College of Pharmacy,

B.V.V.S. Campus, Bagalkot-587101.