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Interspecific hybridisation of lupins

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GRDC project “Interspecific hybridisation of lupins”. UWA00094, 1/1/2006 – 31/12/2008. Staff: Dr Jon Clements (project supervisor, 50%), Mr John Quealy (Research Assistant, 40%), Dr Larissa Prilyuk (40%), Mrs Leah Chong (Research Assistant, 40%).

Lupin breeding in Australia could benefit from the additional genetic diversity of interspecific hybrids. Currently the value of lupins is constrained by several seed quality factors which contribute to their position as a relatively low value feed grain. Improving seed quality of Lupinus angustifolius (narrow-leafed lupin), the most widely adapted species, by increasing protein and oil content or other specific components (e.g. sulphur amino acids) would allow the grain to be sold in a wider range of feed and food markets. Combining desirable attributes among the key crop species (L. angustifolius, L. albus, L. luteus and L. mutabilis) would help to improve an individual species more rapidly. For example a priority is to transfer the seed quality, the brown spot and pleiochaeta root rot tolerance traits from L. luteus to L. angustifolius. Transferring anthracnose resistance, metribuzin tolerance, wide adaptation or aphid tolerance from L. angustifolius to L. luteus would be enormously beneficial in the breeding of yellow lupins. Similarly, transferring high oil and protein characters from pearl lupin (L. mutabilis) to L. angustifolius would be valuable if this were possible. The intended outcomes for the project are:

  • Produce true F1 and backcrossed hybrid lines by focusing on crossing L. angustifolius or L. .luteus with L. .luteus, L. albus and L. mutabilis and additionally L. albus x L. mutabilis.

  • Development of best methods for achieving true hybrids among the crop lupin species including direct hand crossing and seed production on the plant and through embryo rescue.

  • Development of a routine validation of the hybrid nature of the resulting lines by molecular marker methods already in use at DAFWA for lupin breeding programs within species.

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