|RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES KARNATAKA BANGALORE
Dr ASHWINI K.R
FOR THE DEGREE OF AYURVEDA VACHASPATI
TITLE OF THE TOPIC
“COMPARATIVE EVALUATION OF ANTI -INFLAMMATORY ACTIVITY OF
DRONA PUSHPI (Leucas cephalotes Spreng) SWARASA AND KWATHA”
ON ALBINO RATS
NAME OF THE INSTITUTE
Shri B.M.KANKANWADI AYURVEDA MAHAVIDYALAYA
POST GRADUATE STUDIES AND RESEARCH CENTER
Dr ASHWINI K.R.
1st YEAR M.D.
Department of Post Graduate Studies
K.L.E.’s Shri B.M.K. Ayurveda Mahavidyalaya
RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES
JAYANAGAR, 4TH ‘T’ BLOCK, BANGALORE.
THE PRINCIPLE AND HEAD OF THE DEPARTMENT
Post Graduate Studies and Research Center
K.L.E.’s Shri B.M.K.Ayurved Mahavidyalaya
Shahpur, Belgaum 590 003
SUB: Submission of the completed proforma for registration of subject for Dissertation
I request you to kindly register synopsis on below mentioned subject against my name for the submission of dissertation to Rajiv Gandhi University of Health Sciences, Karnataka, Bangalore for partial fulfillment of postgraduate course in the specialization of DravyaGuna M.D. (Ayurveda)
TITLE OF DISSERTATION:
“COMPARATIVE EVALUATION OF ANTI- INFLAMMATORY ACTIVITY OF DRONAPUSHPI (Leucas cephalotes Spreng) SWARASA AND KWATHA”
ON ALBINO RATS
Here with I am enclosing the complete proforma for registration of subject for dissertation.
Date: Dr.Ashwini K.R
RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES KARNATAKA BANGALORE.
COMPLETED PROFORMA FOR REGISTRATION OF SUBJECT FOR DISSERTATION
1. NAME OF THE CANDIDATE : Dr ASHWINI K.R
AND ADDRESS 1st YEAR M.D.
Post Graduate Department of DravyaGuna
K.L.E.’s B.M.K.Ayurved Mahavidyalaya,
2.NAME OF THE INSTITUTE : Shri B.M.K.MAHAVIDYALAYA,
SHAHPUR, BELGAUM-590 003.
3.COURSE OF STUDY : Ayurveda Vachaspati (M.D.) in DravyaGuna
4.DATE OF ADMISSION : 23-06-2008
5. TITLE OF THE TOPIC : COMPARATIVE EVALUATION OF ANTI-
INFLAMMATORY ACTIVITY OF
( Leucas cephalotes Spreng) SWARASA
AND KWATHA ON ALBINO RATS.
6.BRIEF RESUME OF INTENDED WORK:
6.1 NEED FOR STUDY:
“DRONAPUSHPI” (Leucas cephalotes Spreng) of Lamiaceae3 has been described in various Nighantus. Acharya Bhavaprakasha has described Dronapushpi having Shothahara and Bhedana properties4. Same pharmacological activities also supported by Kaiyadeva1 as Shophagna & Shophahara in Madanapala Nigantu9.
So, all the above said references indicate that Dronapushpi possesses Shophahara (Anti-inflammatory). Therefore this study intends to evaluate anti-inflammatory activity of Dronapushpi.
6.2REVIEW OF LITERATURE:
Botanical Name: Leucas cephalotes Spreng.3
Gana: It is not mentioned under any Ganas
An annual hairy pubescent herb, 2-3 feet high.
Root- Cylindrical, zig-zag , smooth, long with numerous wiry, fine rootlets, size variable.
Stem & branches- Light greenish yellow surface, obtusely quadrangular with four prominent furrows, hairy with spreading hairs.
Leaves-1.5 -3 by 0.5-1 inch, Ovate or Ovate lanceolate, subacute, membranous, more or less pubescent, crenate-serrate, base tapering; Petiole 0.25-0.5 inch long.
Flowers- Complete, Regular, Zygomorphic, Bracteate, Sessile, in large globose, dense terminal whorls 1-2 inch in diameter,White
Fruits- Schizocarpic , Carcerule, Nutlets 4,obovoid-oblong,smooth,brownish
Seeds – 0.3 cm long, 3.0-1 cm wide, oblong Trigonous, smooth dark brown in colour.
Rasa – Madhura, Katu, Lavana,
Guna – Guru, Ruksha, Tikshna
Virya – Ushna
Vipaka – Madhura
Dosha-karma – KaphaVatashamaka
Karma- Shothaghna, Jwaraghna, Rechaka, Bhedana, Deepana, Anulomana, Krimighna, Kaphaghna,, Vishaghna, Artavajanana, Swedajanana
Distribution: Found throughout country as a weed. Habitually available in coast of Kathiawar
& Ahamadabad 6, 3.
Chemical Constituent’s-Laballenic acid, B-sitosterol and its Glucoside6
Note (Previous works done): -
1.L.cephalotes extract showed antispasmodic activity 6
2.The LD50 of L.Cephalotes extract in mice was 750mg/kg i.p.6
3.The DL50 of ethanolic extract was found to be 1000mg/kg/i.p6
4.It is found to be effective in the treatment of viral hepatitis.6
5.Distribution of different phytochemical constituents in 5 species of Leucas has been
reported.Leucas cephalotes has been found to maintain a considerable taxonomic distance
with the rest of species and the dendrogram of cluster analysis delineates the same.10
6.From Leucas cephalotes (Lamiaceae), new labdane-, norlabdane-, and abietane- type
diterpenes named leucastrins A and B were isolated, together with a known triterpene, oleonic
acid, 5 sterols and 8 flavones. The structure of new compounds were determined on the basis
of spectral and chemical data.13
6.3 OBJECTIVE OF STUDY:
-To Study the Pharmacognostic & Phyto-chemical evaluation of collected sample of
Dronapushpi and Analysis of its Swarasa and Kwatha.
To evaluate & compare the Anti-inflammatory activity of Dronapushpi Swarasa and kwatha
(In vitro) with the Standard drug.
7. MATERIALS AND METHODS:
7.1 Source of Data:
Collection of “Dronapushpi” (Leucas cephalotes Spreng.) from the Garden of K.L.E.’s
Source of animal: K.L.E.’s B.M.K.Animal House
7.2 Method of collection data:
1. Dronapushpi will be collected from Garden of the K.L.E.’s B.M.K.Ayurved
Mahavidyalaya in the morning hours
2. Dronapushpi Swarasa and Kwatha is prepared as per the Sharangadhara samhitha11.
3. Wistar rats will be divided into Control, Standard and Treatment Groups (n=06s in each group).
Drug will be administered in single dose prior to induction of acute inflammation by injecting carrageenan
(0.1ml of suspension in 0.9% saline) in sub-plantar region. Paw volume changes in ml will be monitored at
0, 0.5, 1, 3, 5 hours after injection carrageenan using plethysmograph.
A) Identification, Authentification of “Dronapushpi”, & Collection
B) Collected samples of “Dronapushpi”(Leucas cephalotes Spreng.)
Will be subjected to following procedure:
Pharmacognostic study of “Dronapushpi”.
Physicochemical study of “Dronapushpi”
Acid soluble/insoluble ash
Water soluble/insoluble ash
Different solvent extraction:
a) Organoleptic analysis of Swarasa and Kwatha
b) Micro-chemical analysis
c) Fluorescence analysis
Qualitative analysis by instrumental method
C) Animal study will be conducted as follow: -
Dose Fixation according to the Sharangadhara samhitha11.
Healthy Wistar rats weighing between 120-150 gms of age 17-20 weeks will be procured and will be maintained in laboratory condition with normal diet and water for 3-5 days and divided into 3 groups each group containing 06 animals
Control group –Distilled water with gum acacia will be given.
Swarasa of test drug will be given
Kwatha of the test drug will be given
Standard drug Aspirin will be given.
Route of Administration: Oral Route
a) Acute inflammation will be induced by injecting carrageenan (0.1ml of 1% suspension in
0.9% saline) in sub-plantar region12.
b) Treatment schedule:
Each rat in test group will be given test drug one hour before being subjected to edema.
c) Observation: -
Paw volume changes in ml be monitored at 0, 0.5, 1, 3 and 5 hours after injecting
carrageenan using plethysmograph12.
d) Results will be analyzed by statistical method (Students t-test and if required ANNOVA test)
7.3 Does the study require any investigations or interventions conducted on animals,
patients or humans? (If so describe briefly)
The experiment will be carried out on individually housed Wistar rat’s colonies that are
maintained on standard pattern of food, water and temperature.
7.4 Has the ethical clearance been obtained from your institution?
8. LIST OF REFERENCES:
1. Sharma P.V. & Sharma G “Kaiyyadeva Nigantu”, Chaukhambha Orientalia,
Varanasi, 1st Edition 1979, Page no.13.
2. Nadkarni K.M, “Indian Materia Medica”, Popular Prakashana Private Limited, III Edition,
Vol-1, Page no.739
Kirtikar.K.R. & Basu.B.D. “Indian Medicinal Plant”,Lalith Mohan Basu Publication,
Allahabad, II Edition, III Vol,Page no.2017
4.Chunekar K.C,“Bhavaprakasha Nighantu” Choukhamba Vishwabharti Academy,
Varanasi, Guduchyadi varga, Page no.463
5.Pandey.G.’ “Dravya-Guna Vijanan” Krishnadas Academy, Varanasi, II Edition
2001, Vol.1, Page no.610
6.Shastry.J.L.N “Dravyaguna Vijananam” Chaukhambha Orientalia, Varanasi, II Edition
2005, Vol.2, Page no.434
7.Pandit Narahari, Raja Nigantu, Chowkhamba Krishnadas Academy, Varanasi, II Edition
8. Sharma P.V, Priya Nigantu, Chowkhamba Prakashan,Varanasi,Edition I,2004, Page No.111
9.Rajavaidya P, Madanapala Nigantu, Khemraj Shrikrishnadas, Mumbai, I Edition, Page No.52
10.Journal of Economic and Taxonomic Botany, Vol-22 (2), 1998, P.N.639-642
11.Pandit Parashurama Shastry, Sharangadhara Samhitha with Deepika Commentary,
Madhyama Khanda, Chowkhamba Orientalia, Varanasi, VI Edition, Page No.137,144
12.M.N.Ghosh, Fundamentals Of The Experimental Pharmacology, Hilton And Company,
Kolkatta, III Edition, Page No.178
13.Chemical and Pharmaceutical Bulletin, Vol-54 (10) 2006, Page No.1370-1379.
9. SIGNATURE OF THE CANDIDATE:
10.REMARKS OF THE GUIDE : The drug Dronaphshpi is one of the
Shophahara drug mentioned in
classics, so the study is planned to
evaluate the Anti-Inflammatory
activity in Wistar Strain Albino Rats
11. NAME AND DESIGNATION OF
11.1 GUIDE : Dr. Yogini R. Kulkarni
M.D.,Ph. D(Ayu),M.A (Sanskrit &Sangeet),D.A & Y.,F.I.I.M
Dept. of Dravya Guna
11.2 SIGNATURE :
11.3 CO-GUIDE : Prof.A.D.Taranalli,
College of Pharmacy,
Nehru Nagar, Belgaum
11.4 SIGNATURE :
11.5 HEAD OF THE DEPARTMENT : Dr.S.K.Hiremath, Prof.
H.O.D.of PG Dept. of Dravya Guna,
12 REMARKS OF THE CHAIRMAN : The above synopsis is scrutinized by
AND PRINCIPAL Guide, HOD,and accorded by Internal
Ethical Committee and
recommended for the registration of
subject for dissertation
12.1 SIGNATURE :