Dr ashwini k. R for the degree of ayurveda vachaspati m. D. Ayurveda in dravya guna

IN

DRAVYA GUNA

K.L.E.SOCIETY’S

Shri B.M.KANKANWADI AYURVEDA MAHAVIDYALAYA

POST GRADUATE STUDIES AND RESEARCH CENTER

SHAHAPUR BELGAUM

FROM:

1^st YEAR M.D.

Department of Post Graduate Studies

In DRAVYAGUNA

K.L.E.’s Shri B.M.K. Ayurveda Mahavidyalaya

Shahpur, Belgaum-590003

TO:

THE REGISTRAR

KARNATAKA

JAYANAGAR, 4^TH ‘T’ BLOCK, BANGALORE.

THROUGH,

IN DRAVYAGUNA

Post Graduate Studies and Research Center

K.L.E.’s Shri B.M.K.Ayurved Mahavidyalaya

Shahpur, Belgaum 590 003
SUB: Submission of the completed proforma for registration of subject for Dissertation

Respected Sir,

I request you to kindly register synopsis on below mentioned subject against my name for the submission of dissertation to Rajiv Gandhi University of Health Sciences, Karnataka, Bangalore for partial fulfillment of postgraduate course in the specialization of DravyaGuna M.D. (Ayurveda)
TITLE OF DISSERTATION:
“COMPARATIVE EVALUATION OF ANTI- INFLAMMATORY ACTIVITY OF DRONAPUSHPI (Leucas cephalotes Spreng) SWARASA AND KWATHA”

ON ALBINO RATS
Here with I am enclosing the complete proforma for registration of subject for dissertation.

Thanking You,

Yours faithfully,
Place: Belgaum

Date: Dr.Ashwini K.R

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES KARNATAKA BANGALORE.

COMPLETED PROFORMA FOR REGISTRATION OF SUBJECT FOR DISSERTATION

1. NAME OF THE CANDIDATE : Dr ASHWINI K.R

AND ADDRESS 1^st YEAR M.D.

Post Graduate Department of DravyaGuna

K.L.E.’s B.M.K.Ayurved Mahavidyalaya,

Shahpur, Belgaum-590003,

Karnataka

2.NAME OF THE INSTITUTE : Shri B.M.K.MAHAVIDYALAYA,

SHAHPUR, BELGAUM-590 003.

3.COURSE OF STUDY : Ayurveda Vachaspati (M.D.) in DravyaGuna

OF COURSE

5. TITLE OF THE TOPIC : COMPARATIVE EVALUATION OF ANTI-

“DRONAPUSHPI”

So, all the above said references indicate that Dronapushpi possesses Shophahara (Anti-inflammatory). Therefore this study intends to evaluate anti-inflammatory activity of Dronapushpi.

Botanical Name: Leucas cephalotes Spreng.³

Family: Lamiaceae³

Gana: It is not mentioned under any Ganas
Morphology^{3, 2}:
An annual hairy pubescent herb, 2-3 feet high.

Root- Cylindrical, zig-zag , smooth, long with numerous wiry, fine rootlets, size variable.

Stem & branches- Light greenish yellow surface, obtusely quadrangular with four prominent furrows, hairy with spreading hairs.

Leaves-1.5 -3 by 0.5-1 inch, Ovate or Ovate lanceolate, subacute, membranous, more or less pubescent, crenate-serrate, base tapering; Petiole 0.25-0.5 inch long.

Flowers- Complete, Regular, Zygomorphic, Bracteate, Sessile, in large globose, dense terminal whorls 1-2 inch in diameter,White

Fruits- Schizocarpic , Carcerule, Nutlets 4,obovoid-oblong,smooth,brownish

Seeds – 0.3 cm long, 3.0-1 cm wide, oblong Trigonous, smooth dark brown in colour.
Properties^1,4,7,8,9:
Rasa – Madhura, Katu, Lavana,

Guna – Guru, Ruksha, Tikshna

Virya – Ushna

Vipaka – Madhura

Dosha-karma – KaphaVatashamaka

Karma- Shothaghna, Jwaraghna, Rechaka, Bhedana, Deepana, Anulomana, Krimighna, Kaphaghna,, Vishaghna, Artavajanana, Swedajanana
Distribution: Found throughout country as a weed. Habitually available in coast of Kathiawar

& Ahamadabad ^{6, 3}.

2.The LD50 of L.Cephalotes extract in mice was 750mg/kg i.p.⁶

3.The DL50 of ethanolic extract was found to be 1000mg/kg/i.p⁶

4.It is found to be effective in the treatment of viral hepatitis.⁶

5.Distribution of different phytochemical constituents in 5 species of Leucas has been

reported.Leucas cephalotes has been found to maintain a considerable taxonomic distance

with the rest of species and the dendrogram of cluster analysis delineates the same.¹⁰

6.From Leucas cephalotes (Lamiaceae), new labdane-, norlabdane-, and abietane- type

diterpenes named leucastrins A and B were isolated, together with a known triterpene, oleonic

acid, 5 sterols and 8 flavones. The structure of new compounds were determined on the basis

of spectral and chemical data.¹³
6.3 OBJECTIVE OF STUDY:

-To Study the Pharmacognostic & Phyto-chemical evaluation of collected sample of

-

To evaluate & compare the Anti-inflammatory activity of Dronapushpi Swarasa and kwatha

7.1 Source of Data:

1. 
   Collection of “Dronapushpi” (Leucas cephalotes Spreng.) from the Garden of K.L.E.’s
   

2. 
   Source of animal: K.L.E.’s B.M.K.Animal House
   

7.2 Method of collection data:

Mahavidyalaya in the morning hours

Drug will be administered in single dose prior to induction of acute inflammation by injecting carrageenan

(0.1ml of suspension in 0.9% saline) in sub-plantar region. Paw volume changes in ml will be monitored at

0, 0.5, 1, 3, 5 hours after injection carrageenan using plethysmograph.

Will be subjected to following procedure:

1. 
   Pharmacognostic study of “Dronapushpi”.
   
   1. 
      Macroscopic Study
      
   2. 
      Microscopic Study
      

2. 
   Physicochemical study of “Dronapushpi”
   
   1. 
      Moisture content
      
   2. 
      Ash value
      
   3. 
      Acid soluble/insoluble ash
      
   4. 
      Water soluble/insoluble ash
      

3. 
   Different solvent extraction:
   
   1. 
      Aqueous extract
      
   2. 
      Alcoholic extract
      

4. 
   a) Organoleptic analysis of Swarasa and Kwatha
   

c) Fluorescence analysis

5. 
   Qualitative analysis by instrumental method
   
   1. 
      T.L.C.
      
   2. 
      H.P.T.L.C
      

C) Animal study will be conducted as follow: -

1. 
   Dose Fixation according to the Sharangadhara samhitha^11.
   

2. 
   Healthy Wistar rats weighing between 120-150 gms of age 17-20 weeks will be procured and will be maintained in laboratory condition with normal diet and water for 3-5 days and divided into 3 groups each group containing 06 animals
   

Route of Administration: Oral Route

a) Acute inflammation will be induced by injecting carrageenan (0.1ml of 1% suspension in

0.9% saline) in sub-plantar region¹².
b) Treatment schedule:
Each rat in test group will be given test drug one hour before being subjected to edema.

c) Observation: -

carrageenan using plethysmograph¹².

maintained on standard pattern of food, water and temperature.

Yes, Enclosed

Varanasi, 1^st Edition 1979, Page no.13.

2. Nadkarni K.M, “Indian Materia Medica”, Popular Prakashana Private Limited, III Edition,

Vol-1, Page no.739

3.

1. 
   Kirtikar.K.R. & Basu.B.D. “Indian Medicinal Plant”,Lalith Mohan Basu Publication,
   

4.Chunekar K.C,“Bhavaprakasha Nighantu” Choukhamba Vishwabharti Academy,

Varanasi, Guduchyadi varga, Page no.463

5.Pandey.G.’ “Dravya-Guna Vijanan” Krishnadas Academy, Varanasi, II Edition

2001, Vol.1, Page no.610
6.Shastry.J.L.N “Dravyaguna Vijananam” Chaukhambha Orientalia, Varanasi, II Edition

2005, Vol.2, Page no.434

Page No.132.

10.Journal of Economic and Taxonomic Botany, Vol-22 (2), 1998, P.N.639-642

11.Pandit Parashurama Shastry, Sharangadhara Samhitha with Deepika Commentary,

Madhyama Khanda, Chowkhamba Orientalia, Varanasi, VI Edition, Page No.137,144

12.M.N.Ghosh, Fundamentals Of The Experimental Pharmacology, Hilton And Company,

Kolkatta, III Edition, Page No.178

13.Chemical and Pharmaceutical Bulletin, Vol-54 (10) 2006, Page No.1370-1379.
9. SIGNATURE OF THE CANDIDATE:

10.REMARKS OF THE GUIDE : The drug Dronaphshpi is one of the

Shophahara drug mentioned in

classics, so the study is planned to

evaluate the Anti-Inflammatory

activity in Wistar Strain Albino Rats

11. NAME AND DESIGNATION OF

11.1 GUIDE : Dr. Yogini R. Kulkarni

Professor

Dept. of Dravya Guna

K.L.E’s B.M.K.Ayurveda

Mahavidyalaya,

Shahpur, Belgaum-590003,

Karnataka

11.2 SIGNATURE :

Vice Principal,

College of Pharmacy,

Nehru Nagar, Belgaum

11.4 SIGNATURE :
11.5 HEAD OF THE DEPARTMENT : Dr.S.K.Hiremath, Prof.

H.O.D.of PG Dept. of Dravya Guna,

K.L.E’s B.M.K.Ayurveda

Mahavidyalaya,

Shahpur, Belgaum-590003,

Karnataka

12 REMARKS OF THE CHAIRMAN : The above synopsis is scrutinized by

AND PRINCIPAL Guide, HOD,and accorded by Internal

Ethical Committee and

recommended for the registration of

subject for dissertation