| Cryptic and repeated ‘allopolyploid’ speciation within Allium przewalskianum Regel. (Alliaceae) from the Qinghai-Tibet Plateau
Organisms Diversity & Evolution
Qianlong Liang, Xiaoxiao Hu, Guili Wu, Jianquan Liu*
*Corresponding author, e-mail: liujq@nwipb.ac.cn, postal address: MOE Key Laboratory for Bio-resources and Eco-environment, College of Life Science, Sichuan University, 24，South Section 1, Yihuan Road, Chengdu 610065, China
Table S1 Details of the Allium przewalskianum diploid–tetraploid complex sampling locations and sample sizes analyzed; P. denotes the population code.
P.
|
Location
|
Latitude(N)
|
Longitude(E)
|
Altitude(m)
|
Sample size(individual number)
|
AFLP
|
ITS
|
CHS
|
1
|
Huzhu, QH
|
37°03′
|
102°24′
|
2378
|
7
|
1
|
1
|
2
|
Ledu, QH
|
36°25′
|
102°46′
|
2560
|
7
|
1
|
1
|
3
|
Xining,QH
|
36°34′
|
101°54′
|
2210
|
8
|
1
|
1
|
4
|
Haiyan,QH
|
36°51′
|
101°03′
|
2950
|
8
|
2
|
1
|
5
|
Lintao,GS
|
35°23′
|
103°50′
|
1880
|
5
|
1
|
2
|
6
|
Luqu,GS
|
34°36′
|
102°28′
|
3120
|
6
|
1
|
1
|
7
|
Tongren,QH
|
35°16′
|
101°53′
|
3170
|
6
|
1
|
1
|
8
|
Guoluo,QH
|
33°43′
|
100°49′
|
3500
|
6
|
1
|
2
|
9
|
Hongyuan,SC
|
33°01′
|
102°36′
|
3470
|
7
|
1
|
1
|
10
|
Shiqu,SC
|
33°00′
|
98°20′
|
3970
|
8
|
2
|
3
|
11
|
Jiangda,XZ
|
31°27′
|
98°09′
|
3640
|
8
|
2
|
1
|
12
|
Yushu,QH
|
31°51′
|
96°18′
|
3660
|
8
|
1
|
2
|
13
|
Lixian,SC
|
31°28′
|
103°07′
|
1954
|
6
|
1
|
1
|
14
|
Aerjinshan,GS
|
38°33′
|
90°10′
|
3358
|
4
|
1
|
1
|
15
|
Dangjinshan,GS
|
39°23′
|
94°20′
|
3930
|
2
|
1
|
1
|
16
|
Yumen,GS
|
39°48′
|
97°17′
|
3361
|
4
|
1
|
1
|
17
|
Delingha,QH
|
37°27′
|
97°20′
|
3310
|
4
|
1
|
1
|
18
|
Xinghai,QH
|
35°53′
|
99°41′
|
3760
|
5
|
1
|
1
|
19
|
Shandan,GS
|
38°06′
|
101°33′
|
2612
|
5
|
1
|
1
|
20
|
Zeku,QH
|
35°12′
|
100°55′
|
3500
|
7
|
1
|
1
|
21
|
Yushu,QH
|
33°51′
|
97°11′
|
4020
|
3
|
1
|
1
|
22
|
Yushu,QH
|
33°00′
|
97°19′
|
3610
|
5
|
1
|
1
|
23
|
Yushu,QH
|
32°21′
|
96°26′
|
3680
|
3
|
1
|
1
|
24
|
Dingqing,XZ
|
31°30′
|
95°23′
|
3770
|
6
|
1
|
1
|
25
|
Leiwuqi,XZ
|
31°12′
|
96°36′
|
3800
|
5
|
1
|
1
|
26
|
Ranwu,XZ
|
29°30′
|
96°46′
|
3950
|
5
|
1
|
1
|
27
|
Dangxiong,XZ
|
30°32′
|
91°08′
|
4310
|
4
|
1
|
1
|
28
|
Kangma,XZ
|
28°33′
|
89°43′
|
4430
|
5
|
1
|
1
|
Note: (1) Abbreviations: QH, Qinghai; GS, Gansu; SC, Sichuan; XZ, Xizang; YN, Yunnan; (2) populations 1-12 are diploid; populations 13-28 are tetraploid and the population codes are in bold.
Table S2 AFLP adapter and primer sequences used in this study
Code
|
Name
|
Sequence
|
E-F
|
EcoR 1-F(adapter)
|
5’-CTCGTAGACTGCGTACC-3’
|
E-R
|
EcoR 1-R(adapter)
|
5’-AATTGGTACGCAGTCTAC-3’
|
M-F
|
Mse 1-F(adapter)
|
5’-GACGATGAGTCCTGAG-3’
|
M-R
|
Mse 1-R(adapter)
|
5’-TACTCAGGACTCAT-3’
|
E-A
|
EcoR 1-A(Primer)
|
5’-GACTGCGTACCAATTCA-3’
|
M-C
|
Mse 1-C(Primer)
|
5’-GATGAGTCCTGAGTAAC-3’
|
E-ACC
|
EcoR 1-ACC(Primer)
|
5’-GACTGCGTACCAATTCACC-3’
|
M-CAC
|
Mse 1-CAC(Primer)
|
5’-GAT GAG TCC TGA GTA ACAC-3’
|
E-ACT
|
EcoR 1-ACT(Primer)
|
5’-GACTGCGTACCAATTCACT-3’
|
M-CTA
|
Mse 1-CTA(Primer)
|
5’-GAT GAG TCC TGA GTA ACTA-3’
|
Table S3 Population codes (P.), plant sources, and locality of the sequenced material; ITS and CHS GenBank accession numbers for all sequences included in this study.
P.
|
Voucher
|
Locality
|
ITS
|
CHS
|
1
|
J. Q. Liu-AO 01
|
China: Huzhu, QH
|
KM189069
|
KM188957
|
1
|
J. Q. Liu-AO 01
|
China: Huzhu, QH
|
KM189070
|
KM188958
|
1
|
J. Q. Liu-AO 01
|
China: Huzhu, QH
|
-
|
KM188959
|
2
|
J. Q. Liu-AO 69
|
China: Ledu, QH
|
KM189071
|
KM188960
|
2
|
J. Q. Liu-AO 69
|
China: Ledu, QH
|
KM189072
|
KM188961
|
2
|
J. Q. Liu-AO 69
|
China: Ledu, QH
|
-
|
KM188962
|
3
|
J. Q. Liu-AO 72
|
China: Xining, QH
|
KM189073
|
KM188963
|
3
|
J. Q. Liu-AO 72
|
China: Xining, QH
|
KM189074
|
KM188964
|
3
|
J. Q. Liu-AO 72
|
China: Xining, QH
|
-
|
KM188965
|
4
|
J. Q. Liu-AO 14
|
China: Haiyan, QH
|
KM189075
|
KM188966
|
4
|
J. Q. Liu-AO 14
|
China: Haiyan, QH
|
KM189076
|
KM188967
|
4
|
J. Q. Liu-AO 14
|
China: Haiyan, QH
|
KM189077
|
-
|
5
|
J. Q. Liu-AO 66
|
China: Lintao, GS
|
KM189078
|
KM188968
|
5
|
J. Q. Liu-AO 66
|
China: Lintao, GS
|
-
|
KM188969
|
5
|
J. Q. Liu-AO 66
|
China: Lintao, GS
|
-
|
KM188970
|
5
|
J. Q. Liu-AO 66
|
China: Lintao, GS
|
-
|
KM188971
|
6
|
J. Q. Liu-2006033
|
China: Luqu, GS
|
KM189079
|
KM188972
|
6
|
J. Q. Liu-2006033
|
China: Luqu, GS
|
-
|
KM188973
|
7
|
J. Q. Liu-AO 54
|
China: Tongren, QH
|
KM189080
|
KM188974
|
7
|
J. Q. Liu-AO 54
|
China: Tongren, QH
|
-
|
KM188975
|
7
|
J. Q. Liu-AO 54
|
China: Tongren, QH
|
-
|
KM188976
|
7
|
J. Q. Liu-AO 54
|
China: Tongren, QH
|
-
|
KM188977
|
7
|
J. Q. Liu-AO 54
|
China: Tongren, QH
|
-
|
KM188978
|
7
|
J. Q. Liu-AO 54
|
China: Tongren, QH
|
-
|
KM188979
|
7
|
J. Q. Liu-AO 54
|
China: Tongren, QH
|
-
|
KM188980
|
8
|
J. Q. Liu-AO 53
|
China: Guoluo, QH
|
KM189081
|
KM188981
|
8
|
J. Q. Liu-AO 53
|
China: Guoluo, QH
|
-
|
KM188982
|
8
|
J. Q. Liu-AO 53
|
China: Guoluo, QH
|
-
|
KM188983
|
8
|
J. Q. Liu-AO 53
|
China: Guoluo, QH
|
-
|
KM188984
|
8
|
J. Q. Liu-AO 53
|
China: Guoluo, QH
|
-
|
KM188985
|
8
|
J. Q. Liu-AO 53
|
China: Guoluo, QH
|
-
|
KM188986
|
9
|
J. Q. Liu-2006026
|
China: Hongyuan, SC
|
KM189082
|
KM188987
|
9
|
J. Q. Liu-2006026
|
China: Hongyuan, SC
|
-
|
KM188988
|
10
|
J. Q. Liu-AO 108
|
China: Shiqu, SC
|
KM189083
|
KM188989
|
10
|
J. Q. Liu-AO 108
|
China: Shiqu, SC
|
KM189084
|
KM188990
|
10
|
J. Q. Liu-AO 108
|
China: Shiqu, SC
|
KM189085
|
KM188991
|
10
|
J. Q. Liu-AO 108
|
China: Shiqu, SC
|
-
|
KM188992
|
10
|
J. Q. Liu-AO 108
|
China: Shiqu, SC
|
-
|
KM188993
|
10
|
J. Q. Liu-AO 108
|
China: Shiqu, SC
|
-
|
KM188994
|
10
|
J. Q. Liu-AO 108
|
China: Shiqu, SC
|
-
|
KM188995
|
11
|
J. Q. Liu-AO 117
|
China: Jiangda, XZ
|
KM189086
|
KM188996
|
11
|
J. Q. Liu-AO 117
|
China: Jiangda, XZ
|
KM189087
|
KM188997
|
11
|
J. Q. Liu-AO 117
|
China: Jiangda, XZ
|
KM189088
|
KM188998
|
11
|
J. Q. Liu-AO 117
|
China: Jiangda, XZ
|
KM189089
|
-
|
12
|
J. Q. Liu-AO 106
|
China: Yushu, QH
|
KM189090
|
KM188999
|
12
|
J. Q. Liu-AO 106
|
China: Yushu, QH
|
KM189091
|
-
|
13
|
J. Q. Liu-TB07024
|
China: Lixian, SC
|
KM189092
|
KM189000
|
13
|
J. Q. Liu-TB07024
|
China: Lixian, SC
|
KM189093
|
KM189001
|
13
|
J. Q. Liu-TB07024
|
China: Lixian, SC
|
-
|
KM189002
|
13
|
J. Q. Liu-TB07024
|
China: Lixian, SC
|
-
|
KM189003
|
13
|
J. Q. Liu-TB07024
|
China: Lixian, SC
|
-
|
KM189004
|
14
|
J. Q. Liu-TB07121
|
China: Aerjinshan, XJ
|
KM189094
|
KM189005
|
14
|
J. Q. Liu-TB07121
|
China: Aerjinshan, XJ
|
-
|
KM189006
|
14
|
J. Q. Liu-TB07121
|
China: Aerjinshan, XJ
|
-
|
KM189007
|
14
|
J. Q. Liu-TB07121
|
China: Aerjinshan, XJ
|
-
|
KM189008
|
14
|
J. Q. Liu-TB07121
|
China: Aerjinshan, XJ
|
-
|
KM189009
|
15
|
J. Q. Liu-TB07117
|
China: Dangjinshan, GS
|
KM189095
|
KM189010
|
15
|
J. Q. Liu-TB07117
|
China: Dangjinshan, GS
|
-
|
KM189011
|
15
|
J. Q. Liu-TB07117
|
China: Dangjinshan, GS
|
-
|
KM189012
|
15
|
J. Q. Liu-TB07117
|
China: Dangjinshan, GS
|
-
|
KM189013
|
16
|
J. Q. Liu-TB07113
|
China: Yumen, GS
|
KM189096
|
KM189014
|
16
|
J. Q. Liu-TB07113
|
China: Yumen, GS
|
-
|
KM189015
|
16
|
J. Q. Liu-TB07113
|
China: Yumen, GS
|
-
|
KM189016
|
16
|
J. Q. Liu-TB07113
|
China: Yumen, GS
|
-
|
KM189017
|
16
|
J. Q. Liu-TB07113
|
China: Yumen, GS
|
-
|
KM189018
|
17
|
J. Q. Liu-AO 160
|
China: Delingha, QH
|
KM189097
|
KM189019
|
17
|
J. Q. Liu-AO 160
|
China: Delingha, QH
|
KM189098
|
KM189020
|
17
|
J. Q. Liu-AO 160
|
China: Delingha, QH
|
-
|
KM189021
|
17
|
J. Q. Liu-AO 160
|
China: Delingha, QH
|
-
|
KM189022
|
17
|
J. Q. Liu-AO 160
|
China: Delingha, QH
|
-
|
KM189023
|
17
|
J. Q. Liu-AO 160
|
China: Delingha, QH
|
-
|
KM189024
|
18
|
J. Q. Liu-AO 82
|
China: Xinghai, QH
|
KM189099
|
KM189025
|
18
|
J. Q. Liu-AO 82
|
China: Xinghai, QH
|
KM189100
|
KM189026
|
18
|
J. Q. Liu-AO 82
|
China: Xinghai, QH
|
-
|
KM189027
|
18
|
J. Q. Liu-AO 82
|
China: Xinghai, QH
|
-
|
KM189028
|
18
|
J. Q. Liu-AO 82
|
China: Xinghai, QH
|
-
|
KM189029
|
19
|
J. Q. Liu-TB07101
|
China: Shandan, GS
|
KM189101
|
KM189030
|
19
|
J. Q. Liu-TB07101
|
China: Shandan, GS
|
KM189102
|
KM189031
|
19
|
J. Q. Liu-TB07101
|
China: Shandan, GS
|
-
|
KM189032
|
19
|
J. Q. Liu-TB07101
|
China: Shandan, GS
|
-
|
KM189033
|
19
|
J. Q. Liu-TB07101
|
China: Shandan, GS
|
-
|
KM189034
|
20
|
J. Q. Liu-AO 41
|
China: Zeku, QH
|
KM189103
|
KM189035
|
20
|
J. Q. Liu-AO 41
|
China: Zeku, QH
|
KM189104
|
KM189036
|
20
|
J. Q. Liu-AO 41
|
China: Zeku, QH
|
-
|
KM189037
|
20
|
J. Q. Liu-AO 41
|
China: Zeku, QH
|
-
|
KM189038
|
20
|
J. Q. Liu-AO 41
|
China: Zeku, QH
|
-
|
KM189039
|
21
|
J. Q. Liu-AO 89
|
China: Yushu, QH
|
KM189105
|
KM189040
|
21
|
J. Q. Liu-AO 89
|
China: Yushu, QH
|
KM189106
|
KM189041
|
21
|
J. Q. Liu-AO 89
|
China: Yushu, QH
|
-
|
KM189042
|
22
|
J. Q. Liu-AO 95
|
China: Yushu, QH
|
KM189107
|
KM189043
|
22
|
J. Q. Liu-AO 95
|
China: Yushu, QH
|
KM189108
|
KM189044
|
22
|
J. Q. Liu-AO 95
|
China: Yushu, QH
|
-
|
KM189045
|
22
|
J. Q. Liu-AO 95
|
China: Yushu, QH
|
-
|
KM189046
|
23
|
J. Q. Liu-AO 101
|
China: Yushu, QH
|
KM189109
|
KM189047
|
23
|
J. Q. Liu-AO 101
|
China: Yushu, QH
|
KM189110
|
KM189048
|
23
|
J. Q. Liu-AO 101
|
China: Yushu, QH
|
-
|
KM189049
|
23
|
J. Q. Liu-AO 101
|
China: Yushu, QH
|
-
|
KM189050
|
24
|
J. Q. Liu-AO 127
|
China: Dingqing, XZ
|
KM189111
|
KM189051
|
24
|
J. Q. Liu-AO 127
|
China: Dingqing, XZ
|
KM189112
|
KM189052
|
24
|
J. Q. Liu-AO 127
|
China: Dingqing, XZ
|
-
|
KM189053
|
24
|
J. Q. Liu-AO 127
|
China: Dingqing, XZ
|
-
|
KM189054
|
25
|
J. Q. Liu-AO 121
|
China: Leiwuqi, XZ
|
KM189113
|
KM189055
|
25
|
J. Q. Liu-AO 121
|
China: Leiwuqi, XZ
|
KM189114
|
KM189056
|
25
|
J. Q. Liu-AO 121
|
China: Leiwuqi, XZ
|
-
|
KM189057
|
26
|
J. Q. Liu-2006290
|
China: Ranwu, XZ
|
KM189115
|
KM189058
|
26
|
J. Q. Liu-2006290
|
China: Ranwu, XZ
|
KM189116
|
KM189059
|
26
|
J. Q. Liu-2006290
|
China: Ranwu, XZ
|
-
|
KM189060
|
26
|
J. Q. Liu-2006290
|
China: Ranwu, XZ
|
-
|
KM189061
|
26
|
J. Q. Liu-2006290
|
China: Ranwu, XZ
|
-
|
KM189062
|
27
|
J. Q. Liu-AO 157
|
China: Dangxiong, XZ
|
KM189117
|
KM189063
|
27
|
J. Q. Liu-AO 157
|
China: Dangxiong, XZ
|
KM189118
|
KM189064
|
27
|
J. Q. Liu-AO 157
|
China: Dangxiong, XZ
|
-
|
KM189065
|
28
|
J. Q. Liu-2006219
|
China: Kangma, XZ
|
KM189119
|
KM189066
|
28
|
J. Q. Liu-2006219
|
China: Kangma, XZ
|
KM189120
|
KM189067
|
28
|
J. Q. Liu-2006219
|
China: Kangma, XZ
|
-
|
KM189068
|
|
Yüklə 0.75 Mb.