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Supporting information Title: a meta-analysis of arbuscular mycorrhizal effects on plants grown under salt stress Journal Name


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Supporting information

Title: A meta-analysis of arbuscular mycorrhizal effects on plants grown under salt stress Journal Name: Mycorrhiza

Murugesan Chandrasekaran1  Sonia Boughattas1,4  Shuijin Hu2  Sang-Hyon Oh3  Tongmin Sa1*



1Department of Environmental and Biological Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea.

2 Department of Plant Pathology, North Carolina State University, Raleigh, NC 27695, USA.

3Department of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA.

4Parasitology Laboratory, Institute Pasteur Tunis, Tunis, Tunisia.

E-mail: tomsa@chungbuk.ac.kr



Contents:

Supporting information S1: Study Coding Glossary

Supporting information S2: Details of plant responses to AMF inoculation under salt stress

Supporting information S3: Details of Sensitivity analysis

Supporting information Notes S1: A list of references included in the meta-analysis

Supporting information S1: Study Coding Glossary

STUDY CODE: Assigned numerical ID for publication included

NUMBER OF STUDIES: Number of studies included in the meta-analysis

PUBLICATION: First author and year of publication, e.g. Evelin et al., 2012

AMF SPECICES: Generic and species name of treatment organism,

e.g. Glomus intraradices

PLANT SPECIES: Generic and species name of plant studied,

e.g. Lycopersicon esculentum

PLANT FAMILY: Taxonomy of different plant species based on broad plant family

LIFE STYLE: The effect of growth variables, such as herbaceous or woody plants

FUNCTIONAL GROUP: AMF inoculation responses to plant functional groups such as grass, herb, forb, shrub, sub-shrub and trees

LIFE CYCLE: Influence of growth variables, such as annual or perennial plants

STUDY SITE: The effect of symbiosis different among study locations, such as field or green house conditions

LEVEL OF STRESS: The effect of stress level experienced by plant in dS/m and mM DURATION: Experimental days/weeks/months.

NON-CATEGORICAL

PREDICTOR VARIABLE: Measures were grouped into shoot, root and total dry weight,

N, P, K and Na uptake, and proline accumulation

UNIT: Measures were reported in different units

SAMPLE SIZE: Number of replicates used for the experimental analysis

Xc: Control mean

SDc: Control standard deviation

Xe: Experimental mean

SDe: Experimental standard deviation

R: Response ratio

(Treatment/Control i.e. with inoculation/without inoculation)

lnR: log response ratio (Effect size calculations with Meta-win v2.1)

Var(lnR): variance of log response ratio

Supporting information S2: Details of plant responses to AMF inoculation under salt stress

Figure legends

Fig. S1 Comparisons between shoot and root ground biomass responses to AMF inoculation under salinity stress within each categorical analysis. Error Bars are means 95% BS CIs. Where the BS CIs do not overlap with zero, the effect size for a parameter is significant at P<0.05

Fig. S2 Effect size of plant species responses to arbuscular mycorrhizal inoculation under salinity stress. Error Bars are means 95% BS CIs. Where the BS CIs do not overlap with zero, the effect size for a parameter is significant at P<0.05. n=number of observations included in meta-analysis

Fig. S3 Effect size of level of salinity stress to arbuscular mycorrhizal inoculated plants. Error Bars are means 95% BS CIs. Where the BS CIs do not overlap with zero, the effect size for a parameter is significant at P<0.05. n=number of observations included in meta-analysis

Fig. S4 Effect size of growth site (green house vs. field) and duration’s responses to arbuscular mycorrhizal inoculation under salinity stress. Error Bars are means 95% BS CIs. Where the BS CIs do not overlap with zero, the effect size for a parameter is significant at P<0.05. n=number of observations included in meta-analysis

Table S1 Raw data in Excel format used in the meta-analysis

Analysis 1 (All studies), Analysis 2 (Shoot biomass), Analysis 3 (Root biomass), Analysis 4 (Total biomass), Analysis 5 (P uptake), Analysis 6 (N uptake), Analysis 7 (K uptake), Analysis 8 (Na uptake), Analysis 9 (Proline accumulation) and Analysis 10 (Antioxidant enzymes).



Fig. S1 Comparisons between shoot and root biomass responses to AMF inoculation under salinity stress within each categorical analysis. Error Bars are means 95% BS CIs. Where the BS CIs do not overlap with zero, the effect size for a parameter is significant at P<0.05. White box-shoot biomass; Black-filled box-root biomass.



Fig. S2 Effect size of plant species responses to arbuscular mycorrhizal inoculation under salinity stress. Error Bars are means 95% BS CIs. Where the BS CIs do not overlap with zero, the effect size for a parameter is significant at P<0.05. n=number of observations included in meta-analysis.




Fig. S3 Effect size of level of salinity stress to arbuscular mycorrhizal inoculated plants. Error Bars are means 95% BS CIs. Where the BS CIs do not overlap with zero, the effect size for a parameter is significant at P<0.05. n=number of observations included in meta-analysis.



Fig. S4 Effect size of growth site (green house vs. field) and duration’s responses to arbuscular mycorrhizal inoculation under salinity stress. Error Bars are means 95% BS CIs. Where the BS CIs do not overlap with zero, the effect size for a parameter is significant at P<0.05. n=number of observations included in meta-analysis.



Supporting information S3: Details of Sensitivity analysis

Sensitivity Analysis

A sensitivity analysis was conducted to test for any disproportional impact on studies. However, we only applied this procedure on independent categorical variables significantly affecting AMF inoculation under salt stress (shoot biomass, root biomass, N, P, K and Na uptake and antioxidant enzymes dataset). The sensitivity analysis was done in MetaWin by sequentially excluding one study at a time from the dataset. After excluding a study, a new random effects meta-analysis was performed and the effect size estimate and 95% BS CIs were compared with those of the complete dataset. Effect size estimates and 95% BS CIs for each level of the significant categorical independent variables were also investigated.

If the BS CIs did not include the effect size estimate of the complete dataset, then this specific study had a disproportional impact. Consequently, the meta-analysis of the complete dataset had to be repeated without this specific study.

Dataset: complete



Fig. S3a. Sensitivity analysis of complete dataset on effect of AMF inoculation under salt stress. Effect size and 95% BS CIs were presented for all (overall effect with no study excluded) and sequentially exclusion of one study. The values on the x-axes represented study ID of excluded study. No study with disproportional impact was detectable.

Dataset: shoot biomass





Fig. S3b. Sensitivity analysis of shoot biomass dataset. Effect size and 95% BS CIs were presented for all (overall effect with no study excluded) and sequentially exclusion of one study. The values on the x-axes represented study ID of excluded study. No study with disproportional impact was detectable.

Dataset: root biomass





Fig. S3c. Sensitivity analysis of root biomass dataset. Effect size and 95% BS CIs were presented for all (overall effect with no study excluded) and sequentially exclusion of one study. The values on the x-axes represented study ID of excluded study. No study with disproportional impact was detectable.

Dataset: N uptake





Fig. S3d. Sensitivity analysis of N uptake dataset. Effect size and 95% BS CIs were presented for all (overall effect with no study excluded) and sequentially exclusion of one study. The values on the x-axes represented study ID of excluded study. No study with disproportional impact was detectable.

Dataset: P uptake





Fig. S3e. Sensitivity analysis of P uptake dataset. Effect size and 95% BS CIs were presented for all (overall effect with no study excluded) and sequentially exclusion of one study. The values on the x-axes represented study ID of excluded study. No study with disproportional impact was detectable.

Dataset: K uptake





Fig. S3f. Sensitivity analysis of K uptake dataset. Effect size and 95% BS CIs were presented for all (overall effect with no study excluded) and sequentially exclusion of one study. The values on the x-axes represented study ID of excluded study. No study with disproportional impact was detectable.

Dataset: Na uptake





Fig. S3g. Sensitivity analysis of Na uptake dataset. Effect size and 95% BS CIs were presented for all (overall effect with no study excluded) and sequentially exclusion of one study. The values on the x-axes represented study ID of excluded study. No study with disproportional impact was detectable.

Dataset: antioxidant enzymes





Fig. S3h. Sensitivity analysis of antioxidant enzymes dataset. Effect size and 95% BS CIs were presented for all (overall effect with no study excluded) and sequentially exclusion of one study. The values on the x-axes represented study ID of excluded study. No study with disproportional impact was detectable.

Supporting information Notes S1:

A list of references included in the meta-analysis

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