Supplementary Table 10. Analysis of variance of effects of NAA foliar spraying on auxin, zeatin contents (ng/g FW) and MdPIN gene expression in Fuji/M9/Baleng Crab. Means sharing the same letter are not significantly different (P > 0.05) between lines; means sharing the same letter in the brackets are not significantly different between rows.
Detection index
|
Time of treatment
|
part
|
leaf
|
Stem bark
of scion
|
Stem bark of interstock
|
Stem bark of rootstock
|
root
| |
IAA
|
0day
|
218.6565 a
|
139.6750 a
|
151.0189 a
|
101.5154 a
|
114.0190 a
|
1day
|
159.4481 b
|
61.5070 a
|
78.5162 de
|
48.4730 c
|
62.2543 b
|
2day
|
147.5859 b
|
54.8716 bc
|
57.5952 e
|
35.3030 c
|
65.1734 b
|
3day
|
100.0092 c
|
38.2576 c
|
88.6307 cd
|
33.6404 c
|
71.5438 b
|
1week
|
93.1693 c
|
35.5370 c
|
115.2329 b
|
47.5751 c
|
63.9902 b
|
2week
|
72.6389 c
|
62.6823 b
|
109.1835 bc
|
76.8066 b
|
82.3106 b
|
3week
|
164.7884 b
|
70.6617 b
|
122.5626 b
|
96.4406 a
|
109.7617 a
|
IAA total
|
|
|
66.1703(b)
|
103.2486(a)
|
62.8220(b)
|
|
NAA
|
0day
|
0.0000 c
|
0.0000 e
|
0.0000 c
|
0.0000 c
|
0.0000 c
|
1day
|
489.9621 a
|
141.0273 c
|
274.5885 a
|
77.6630 a
|
75.2210 a
|
2day
|
542.6839 a
|
178.0681 b
|
251.3778 a
|
86.7113 a
|
64.5620 a
|
3day
|
520.3038 a
|
220.1249 a
|
242.8154 a
|
74.8526 a
|
67.1668 a
|
1week
|
492.7942 a
|
151.8927 bc
|
277.1460 a
|
76.2099 a
|
71.2995 a
|
2week
|
323.6557 b
|
70.8036 d
|
108.9237 b
|
71.5035 a
|
56.8023 a
|
3week
|
48.7421 c
|
14.1546 c
|
15.8736 c
|
30.0211 b
|
29.9669 b
|
NAA total
|
|
|
110.8673(b)
|
167.2464(a)
|
59.5659(b)
|
|
NAA+IAA
|
0day
|
218.6565 c
|
139.6750 d
|
151.0189 e
|
101.5154 b
|
114.0190 a
|
1day
|
649.4101 a
|
202.5342 bc
|
353.1047 b
|
126.1359 ab
|
137.4753 a
|
2day
|
690.2698 a
|
232.9397 ab
|
308.9730 c
|
122.0143 ab
|
129.7354 a
|
3day
|
620.3129 a
|
258.3825 a
|
331.4461 bc
|
108.4930 b
|
138.7107 a
|
1week
|
585.9635 a
|
187.4296 c
|
392.3789 a
|
123.7850 ab
|
135.2898 a
|
2week
|
396.2945 b
|
133.4859 d
|
218.1072 d
|
148.3102 a
|
139.1130 a
|
3week
|
213.5305 c
|
84.8164 e
|
138.4362 e
|
126.4617 ab
|
139.7286 a
|
NAA+IAA total
|
|
|
177.0376(b)
|
270.4950(a)
|
122.3879(c)
|
| |
Z
|
0day
|
48.6314 a
|
|
|
|
52.5931 ab
|
1day
|
44.5831 a
|
|
|
|
43.3102 b
|
2day
|
51.3024 a
|
|
|
|
62.9454 a
|
3day
|
55.4590 a
|
|
|
|
65.5533 a
|
1week
|
52.1428 a
|
|
|
|
67.0140 a
|
2week
|
51.2389 a
|
|
|
|
58.0613 ab
|
3week
|
48.3501 a
|
|
|
|
57.0580 ab
|
MdPIN8
|
0day
|
|
1.0014 d
|
0.2315 d
|
1.0535 e
|
|
1day
|
|
6.6161 c
|
0.8736 b
|
1.5925 bc
|
|
2day
|
|
13.6554 b
|
0.8692 b
|
1.3299 cde
|
|
3day
|
|
15.3324 ab
|
0.9264 b
|
1.6395 b
|
|
1week
|
|
15.9455 a
|
0.8119 b
|
2.0660 a
|
|
2week
|
|
1.4889 d
|
1.2134 a
|
1.2391 de
|
|
3week
|
|
1.1376 d
|
0.3965 c
|
1.4658 bcd
|
|
MdPIN8 total
|
|
|
7.8825(a)
|
0.7604(b)
|
1.4838(b)
|
|
MdPIN10
|
0day
|
|
0.5300 d
|
0.2434 d
|
0.5351 cd
|
|
1day
|
|
2.2185 c
|
0.8775 c
|
0.6024 c
|
|
2day
|
|
6.1426 a
|
1.0031 bc
|
1.0978 a
|
|
3day
|
|
6.2039 a
|
0.9825 bc
|
0.9955 b
|
|
1week
|
|
3.3820 b
|
1.2188 ab
|
0.6235 c
|
|
2week
|
|
1.1515 d
|
1.4187 a
|
0.4840 d
|
|
3week
|
|
0.5316 d
|
0.1853 d
|
0.9956 b
|
|
MdPIN10 total
|
|
|
2.8800(a)
|
0.8470(b)
|
0.7620(b)
|
| Supplementary Table 11. Analysis of variance of effects of NAA root application on auxin and zeatin contents (ng/g FW) in Fuji/M9/Baleng Crab. Means sharing the same letter are not significantly different (P > 0.05) between lines.
Detection index
|
Time of treatment
|
part
|
leaf
|
root
|
IAA
|
0day
|
204.7031 a
|
99.0144 a
|
1day
|
195.3531 a
|
94.5479 ab
|
2day
|
84.3692 bc
|
69.3944 ab
|
3day
|
73.4785 c
|
61.7766 b
|
1week
|
99.3929 bc
|
73.6678 ab
|
2week
|
102.9120 bc
|
79.9451 ab
|
3week
|
113.5253 b
|
91.9660 ab
|
Z
|
0day
|
53.9117 b
|
57.4586 c
|
1day
|
67.9676 b
|
73.5594 c
|
2day
|
88.2649 a
|
108.2057 b
|
3day
|
98.4562 a
|
125.9003 ab
|
1week
|
101.1748 a
|
142.4478 a
|
2week
|
106.3056 a
|
141.0385 a
|
3week
|
93.6104 a
|
112.1246 b
|
Supplementary Table 12. Analysis of variance of effects of NPA treatment on auxin and zeatin contents (ng/g FW) in Fuji/Baleng Crab. Means sharing the same letter are not significantly different (P > 0.05) between lines.
Detection index
|
Treatment method
|
Time after treatment
|
part
|
leaf
|
Stem bark above treatment area
|
Stem bark beneath treatment area
|
root
|
IAA
|
NPA
|
0day
|
471.9387 a
|
229.3209 b
|
230.6963 a
|
233.9786 a
|
1day
|
461.9197 a
|
427.8144 a
|
53.2854 b
|
181.1085 b
|
2day
|
199.1475 b
|
130.6711 c
|
33.3631 b
|
157.2288 b
|
3day
|
149.7278 bc
|
110.8501 c
|
36.8908 b
|
112.9874 c
|
1week
|
106.3102 c
|
103.5039 c
|
32.4313 b
|
103.9432 c
|
2week
|
83.9551 c
|
89.2687 c
|
44.0233 b
|
109.5138 c
|
3week
|
107.5889 c
|
105.6352 c
|
47.0176 b
|
123.2970 c
|
Z
|
0day
|
100.84071 a
|
|
|
112.00524 a
|
1day
|
93.36732 ab
|
|
|
98.43782 ab
|
2day
|
102.90259 a
|
|
|
108.42505 a
|
3day
|
73.54382 bc
|
|
|
84.63797 bc
|
1week
|
65.29733 c
|
|
|
66.96544 cd
|
2week
|
25.53826 d
|
|
|
49.38256 d
|
3week
|
32.32311 d
|
|
|
54.21114 d
|
IAA
|
lanolin
|
0day
|
489.5190 a
|
230.4692 a
|
220.7161 a
|
229.4121 ab
|
1day
|
417.7304 a
|
228.5762 a
|
212.3171 a
|
246.4058 ab
|
2day
|
506.4476 a
|
216.4816 a
|
168.2156 b
|
239.3606 ab
|
3day
|
461.4684 a
|
174.0967 b
|
143.6547 b
|
221.9507 b
|
1week
|
465.2333 a
|
162.8843 b
|
147.0717 b
|
275.8259 a
|
2week
|
450.8516 a
|
142.6741 b
|
141.9748 b
|
261.6550 ab
|
3week
|
429.7618 a
|
175.6850 b
|
137.5651 b
|
232.7197 ab
|
Z
|
0day
|
99.3656 a
|
|
|
112.0052 a
|
1day
|
98.2091 a
|
|
|
103.4869 a
|
2day
|
95.9298 a
|
|
|
118.3919 a
|
3day
|
107.8855 a
|
|
|
104.7002 a
|
1week
|
101.1748 a
|
|
|
107.8097 a
|
2week
|
106.3056 a
|
|
|
118.5216 a
|
3week
|
105.4532 a
|
|
|
120.3227 a
|
Supplementary Table 13. Analysis of variance of effects of growth regulators treatment on total length, number, and average length of new shoots in apple trees with different rootstocks. *Significantly different (P ≤ 0.05) compared to the control.
variety
|
Treatment method
|
Detection index
|
Total shoot length(cm)
|
Shoot number
|
Average Shoot length(cm)
|
Fuji/M9
|
control
|
137.8
|
6
|
22.5
|
NAA(S)
|
218.05
|
7.5
|
29.7
|
NAA(R)
|
226.65
|
9.5
|
26.3
|
6BA(S)
|
418.1*
|
11.5*
|
36.9 *
|
6BA(R)
|
422.85*
|
10
|
43.3 *
|
Fuji/M9/Baleng Crab
|
control
|
114.8
|
4
|
28.7
|
NAA(S)
|
211.7*
|
6.5*
|
32.7
|
NAA(R)
|
339.9*
|
8*
|
42.7
|
Fuji/Baleng Crab
|
control
|
397.9
|
14
|
28.5
|
NPA
|
110.3*
|
6.5*
|
15.8
|
lanolin
|
388.4
|
10
|
38.9 *
| |
Yüklə 333.5 Kb.