Supplementary Table 6. Analysis of variance of effects of NAA foliar spraying on auxin and zeatin contents (ng/g FW), and MdPIN gene expression in Fuji/M9. Means sharing the same letter are not significantly different (P > 0.05) between lines; NS, not significantly different; and * Significantly different (P ≤ 0.05) between rows.
Detection index
|
Time of treatment
|
part
|
leaf
|
Stem bark of scion
|
Stem bark of rootstock
|
root
|
IAA
|
0day
|
195.9513 a
|
127.4514 a
|
134.1360 a
|
93.1923 ab
|
1day
|
133.4050 b
|
63.7935 b
|
51.9150 bc
|
60.7472 b
|
2day
|
104.3394 bc
|
54.6798 bc
|
36.6851 c
|
67.2793 b
|
3day
|
74.8236 cd
|
36.9042 bc
|
39.8112 c
|
64.9486 b
|
1week
|
69.4579 d
|
33.6636 c
|
31.5344 c
|
58.4471 b
|
2week
|
58.3764 d
|
59.1968 bc
|
52.1713 bc
|
80.7992 ab
|
3week
|
112.8311 b
|
63.0882 b
|
70.8997 b
|
110.3036 ab
|
Z
|
0day
|
47.4804 bc
|
|
|
51.6127 b
|
1day
|
43.5597 c
|
|
|
76.2820 ab
|
2day
|
70.5195 a
|
|
|
73.8902 ab
|
3day
|
75.7325 a
|
|
|
81.4414 a
|
1week
|
59.5036 abc
|
|
|
61.8679 ab
|
2week
|
66.5354 ab
|
|
|
53.0127 b
|
3week
|
47.6630 bc
|
|
|
55.3645 ab
|
MdPIN8
|
0day
|
|
1.0286 d
|
0.5581 d
|
|
1day
|
|
7.4914 bc
|
0.9790 c
|
|
2day
|
|
8.7145 a
|
1.0110 c
|
|
3day
|
|
8.3060 ab
|
1.2489 b
|
|
1week
|
|
7.1768 c
|
2.4328 a
|
|
2week
|
|
2.0372 d
|
0.5303 d
|
|
3week
|
|
1.0538 d
|
0.6162 d
|
|
MdPIN8 total
|
|
|
5.1155 *
|
1.0538
|
|
MdPIN10
|
0day
|
|
1.9027 c
|
0.5345 b
|
|
1day
|
|
6.0681 a
|
1.1516 a
|
|
2day
|
|
6.8804 a
|
1.0975 a
|
|
3day
|
|
6.3580 a
|
1.1957 a
|
|
1week
|
|
4.7867 b
|
1.0645 a
|
|
2week
|
|
1.7852 c
|
0.7432 b
|
|
3week
|
|
1.0272 c
|
0.6152 b
|
|
MdPIN10 total
|
|
|
4.1155 *
|
0.9146
|
|
Supplementary Table 7. Analysis of variance of effects of root application of NAA on auxin and zeatin contents (ng/g FW) in Fuji/M9. Means sharing the same letter are not significantly different (P > 0.05) between lines.
Detection index
|
Time of treatment
|
part
|
leaf
|
root
|
IAA
|
0day
|
172.2863 a
|
91.5163 a
|
1day
|
172.2531 a
|
519.4435 a
|
2day
|
68.3828 b
|
651.3730 a
|
3day
|
70.0362 b
|
490.8261 a
|
1week
|
90.1623 b
|
497.0677 a
|
2week
|
75.9273 b
|
363.8485 a
|
3week
|
84.0586 b
|
180.3076 a
|
Z
|
0day
|
52.3459 c
|
53.9117 c
|
1day
|
72.0292 ab
|
67.9676 bc
|
2day
|
77.4730 a
|
82.9845 ab
|
3day
|
65.9931 abc
|
83.3695 ab
|
1week
|
51.9311 c
|
86.0880 a
|
2week
|
57.9594 bc
|
83.6755 ab
|
3week
|
58.9098 abc
|
86.0670 a
|
Supplementary Table 8. Analysis of variance of effects of 6BA foliar spraying on auxin and zeatin contents (ng/g FW) in Fuji/M9. Means sharing the same letter are not significantly different (P > 0.05) between lines.
Detection index
|
Time of treatment
|
part
|
leaf
|
root
|
Z
|
0day
|
53.2995 a
|
58.0204 a
|
1day
|
55.2931 a
|
64.8854 a
|
2day
|
37.9796 b
|
42.8589 b
|
3day
|
30.5697 bc
|
37.4957 b
|
1week
|
29.6210 bc
|
32.4959 bc
|
2week
|
18.1840 c
|
21.9034 c
|
3week
|
18.8313 c
|
21.5657 c
|
IAA
|
0day
|
169.5459 a
|
89.8136 c
|
1day
|
182.2230 a
|
90.6163 c
|
2day
|
186.9656 a
|
93.6757 c
|
3day
|
192.5047 a
|
148.9749b
|
1week
|
193.0755 a
|
179.6763 b
|
2week
|
194.1121 a
|
222.4733 a
|
3week
|
190.9273 a
|
223.8417 a
|
Supplementary Table 9. Analysis of variance of effects of 6BA root application on auxin and zeatin contents (ng/g FW) in Fuji/M9. Means sharing the same letter are not significantly different (P > 0.05) between lines.
Detection index
|
Time of treatment
|
part
|
leaf
|
root
|
Z
|
0day
|
53.8119 a
|
61.7965 a
|
1day
|
39.1101 ab
|
43.2286 b
|
2day
|
33.5967 bc
|
38.0465 bc
|
3day
|
25.6383 bc
|
24.7230 cd
|
1week
|
25.6671 bc
|
25.2526 cd
|
2week
|
23.2996 bc
|
24.9511 cd
|
3week
|
19.5667 c
|
20.2600 d
|
IAA
|
0day
|
172.0411 a
|
91.5265 cd
|
1day
|
177.6527 a
|
92.0832 cd
|
2day
|
169.4316 a
|
111.7605 c
|
3day
|
183.6019 a
|
78.2682 d
|
1week
|
168.4477 a
|
109.1842 cd
|
2week
|
183.9336 a
|
183.6844 b
|
3week
|
177.8512 a
|
217.6517 a
| |
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