Supplemental Materials: In situ phylogenetic structure and diversity of wild Bradyrhizobium communities

1. 
   MAG Media Reagents (per liter)
   

1.3g HEPES

1.0g Arabinose (DL)

1.0g Gluconic Acid~Sodium Salt)

1.0g Yeast extract

2ml KH₂PO₄ Solution (110g/L)

4ml Na₂SO₄ Solution (62.5 g/L)

1ml MgSO₄ • 7H₂O Solution (180g/L)

2ml NH₄Cl Solution (160g/L)

1ml CaCl₂ Solution (13g/L)

1ml FeCl₃ • 6H₂O Solution (6.7g/L)

Adjust pH to 6.6 with KOH

Autoclave for 40 minutes

Supplemental Tables

Supplemental Table 1. Plant numbers and species collected at each unique GPS site
Site GPS Coordinates Plants Collected

1 38^o19'070 N, 123^o03'560 W L. heermannii 15 and 17, L. strigosus 14 and 16

2 38^o19'100 N, 123^o03'440 W L. strigosus 20-25

3 38^o19'158 N, 123^o03'820 W L. strigosus 7, 8 and 11

4 38^o19'158 N, 123^o03'824 W L. strigosus 1-4

5 38^o20'560 N, 123^o03'566 W L. heermannii 33

6 38^o20'570 N, 123^o03'459 W L. heermannii 34

7 38^o20'571 N, 123^o03'460 W L. micranthus 28

8 38^o20'602 N, 123^o03'458 W L. micranthus 30

9 38^o20'623 N, 123^o03'495 W L. micranthus 27

10 38^o20'642 N, 123^o03'504 W L. micranthus 26

11 38^o20'649 N, 123^o03'514 W L. micranthus 29

Supplemental Table 2. Number of isolates and sequenced genotypes from different microenvironments and hosts.

B. Samples within each host species

Table 3. Sampling type and spatial location of all sequenced isolates. Isolate source = year (05=2005), plant species (LoA = Lotus angustissimus, LoM = L. micranthus, LoH = L. heermannii, LoS = L. strigosus), and nodule or root surface numbers. Genotype ID = isolate name or GenBank accession when unique, multiply recovered genotypes are named with the host species (A, M, H, S), isolate type(s) (N = nodule, R = root surface) and the total number of isolates that share the genotype. Type = isolation type (nod = nodule, old = mature portion of the root surface, tip = root surface tip. Lat = latitude and Long = longitude. Nod =Nodulation status (Y = positive nodulation in all five test plants, N = lack of nodulation in all five test plants, ‘-‘ = untested). Genbank = GenBank Accession number.