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Morphological and allozyme variation of


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APPENDIX 1


Measurements
Wing variables. Forearm (FA); second digit metacarpal (IIMC); first phalanx (IIF1); second phalanx (IIF2); third digit metacarpal (IIIMC); first phalanx (IIIF1); second phalanx (IIIF2); fourth digit metacarpal (IVMC); first phalanx (IVF1); second phalanx (IVF2); fifth digit metacarpal (VMC); first phalanx (VF1); second phalanx (VF2).
Cranial variables. Greatest skull length (GSL); condylo-basal length (CBL); rostrum length (RL); palatal length (PL); zygomatic breadth (ZB); interorbital breadth (IOB); braincase breadth (BCB); toothrow length (C1–M1); breadth across upper canines (C1–C1); breadth across M1–M1 (M1–M1); mandible length (ML); mandible height (MH); mandibular toothrow length (C1–M2); mandibular angle (MA).
Dental variables. Length of P3 (LP3); length of P4 (LP4); length of M1 (LM1); length of M2 (LM2); breadth of P3 (BP3); breadth of P4 (BP4); breadth of M1 (BM1); breadth of M2 (M2); length of P3 (LP3); length of P4 (LP4); length of M1 (LM1); length of M2 (LM2); length of M3 (LM3); breadth of P3 (BP3); breadth of P4 (BP4); breadth of M1 (BM1); breadth of M2 (BM2); breadth of M3 (BM3).




APPENDIX 2

Selected measurements in ∪∪ (Var) of adult Eidolon helvum from the Gulf of Guinea by sex and population. The last three columns correspond to the subspecies



E. h. sabaeum according to Bergmans (1990). Sample sizes are given in parentheses.





R´ıo Muni

∪(13–18), ℑ(11–13)



Bioko

∪(13–15), ℑ(11)



Pr´ıncipe

∪(20–21), ℑ(6–8)



Sa˜ o Tome´

∪(6–9), ℑ(8–10)






Annobo´ n

∪(6–9), ℑ(8)



Saudi Arabia

∪(3–5), ℑ(3–4)



Var

Sex

Min Max Mean

Min Max Mean

Min Max Mean

Min Max Mean

Min

Max Mean

Min Max Mean

FA

∪∪

117.0

129.0

121.7

117.0

132.2

122.9

114.9

125.5

120.1

116.4

124.0

121.0

109.0

115.0

111.7

109.0

124.7

118.0




ℑℑ

118.5

129.0

124.5

116.2

134.0

126.1

118.0

127.0

122.0

114.3

125.0

120.9

107.6

119.0

113.9

113.5

124.3

116.6

GSL

∪∪

52.9

59.4

56.3

52.2

58.7

56.6

51.8

56.6

54.3

51.3

57.9

54.5

49.9

57.0

51.7

52.5

53.6

53.3




ℑℑ

53.8

58.1

56.1

55.6

59.1

56.9

51.8

56.2

54.3

51.9

57.0

54.0

49.5

51.8

50.6

50.4

53.3

52.1

CBL

∪∪

51.3

57.0

54.2

50.1

56.8

54.5

50.1

55.1

52.4

49.9

56.4

52.8

47.4

54.9

49.8

49.9

52.2

51.1




ℑℑ

49.9

55.8

53.6

53.4

57.1

55.1

50.2

54.4

56.5

50.3

54.9

51.8

47.1

49.9

48.7

48.0

51.3

49.6

RL

∪∪

19.1

23.9

21.6

18.8

22.5

21.6

19.5

22.3

20.7

19.8

22.8

21.0

19.0

22.8

20.1










ℑℑ

19.3

22.8

21.6

21.1

23.2

22.1

19.5

21.3

20.7

19.6

21.3

20.3

19.0

20.0

19.6





19.6

PL

∪∪

25.5

29.4

27.4

26.0

29.6

27.6

24.9

27.9

26.6

25.5

28.5

27.1

24.4

27.5

25.6










ℑℑ

26.0

29.0

27.5

26.0

29.5

27.8

25.6

27.7

26.6

25.0

27.6

26.2

24.6

25.8

25.1





28.5

ZB

∪∪

30.8

35.3

33.7

32.6

35.4

33.6

31.1

34.8

32.3

29.8

33.2

32.0

28.7

31.7

30.0

30.3

32.4

31.4




ℑℑ

31.0

33.8

32.2

31.6

34.8

33.0

31.0

32.3

31.6

28.9

32.9

31.4

27.1

29.8

29.0

29.6

31.7

30.7

C1-M2

∪∪

19.1

23.6

21.0

19.7

23.1

21.3

18.7

21.9

20.4

20.1

22.2

20.9

19.0

22.0

20.1

19.5

20.2

19.8




ℑℑ

19.8

22.3

21.2

19.9

22.0

21.2

20.0

21.5

20.7

19.1

21.5

20.4

18.7

19.9

19.3

18.6

19.5

19.1

ML

∪∪

40.5

47.5

44.9

42.7

47.3

45.2

42.0

45.5

43.5

41.4

46.5

43.8

39.8

45.5

41.3

41.3

43

42




ℑℑ

42.1

46.8

44.9

44.2

47.6

45.6

42.8

44.1

43.6

40.7

45.5

43.0

39.0

41.3

40.2

40.5

42.7

41.8

APPENDIX 3


Allozyme systems examined and staining procedures used in the study. Names follow the No- menclature Committee of the International Union of Biochemistry (1984). Each protein is followed by its abbreviation, its Enzyme Commission Number, the tissue examined (H=heart; L=liver), and the buffer utilized. Buffers (TC=tris/citrate; TME=tris/maleate/EDTA; TBE=tris/borate/EDTA) follow Pasteur et al. (1987), except for TC 8, TME 6.9 and LiOH 8.3 which are half diluted for gels.

Aspartate aminotransferase (Aat-1, Aat-2) 2.6.1.1; H/L; TME 6.9. Aconitate hydratase (Acon) 4.2.1.3; H; TC 6.7. Acid phosphatase (Acp) 3.1.3.2; L; TME 6.9/TC 6.4. Adenylate kinase (Ak) 2.7.4.3; H; TC 6.4. Alcohol dehydrogenase (Adh) 1.1.1.1; L; TC 6.4. Carbonic anhydrase (Car) 4.2.1.1; L; TBE 8.6. Creatine kinase (Ck-1, Ck-2) 2.7.3.2; H; TC 6.4. Esterase (Est-1) 3.1.1.1; L; LiOH 8.3. Esterase (Est-



2) 3.1.1.1; L; LiOH 8.3. Fumarate hydratase (Fum) 4.2.1.2; H; TME 6.9. Glycerol-3-phosphate dehydrogenase (c-Gpd-1) 1.1.1.8; H; TC 8/TBE 8.6. Glycerol-3-phosphate dehydrogenase (c-Gpd-2)

1.1.1.8; L; TBE 8.6. Glucose-6-phosphate isomerase (Gpi) 5.3.1.9; H; TME 6.9. Haemoglobine (Hbb); TBE 8.6. Isocitrate dehydrogenase (Idh-1, Idh-2) 1.1.1.42; H/L; TC 6.7. Leucinamide peptidase (Lap)



3.4.11.1; L; TBE 8.6. Lactate dehydrogenase (Ldh-1, Ldh-2) 1.1.1.27; H; TC 6.7. Malate dehydrogenase (Mdh-1, Mdh-2) 1.1.1.37; H/L; TC 6.7. Malic enzyme (Me-1, Me-2) 1.1.1.40; H; TC 6.7. Mannose-6- phosphate isomerase (Mpi) 5.3.1.8; H/L; TBE 8.6. Purine nucleoside phosphorylase (Np) 2.4.2.1; H; TBE 8.6 and L; LiOH 8.3. Leucyl-tyrosine peptidase (Pep-A) 3.4.11; H; TC 8. Leucyl-glycyl-glycine peptidase (Pep-B) 3.4.11; H; TC 8. Phenyl-proline peptidase (PepD) 3.4.11; H; TC 8. 6-Phosphogluconic acid dehydrogenase (6Pgd) 1.1.1.44; H; TC 6.4. Phosphoglucomutase (Pgm-1, Pgm-2) 2.7.5.1; H/L; TC 6.4/TME 6.9. Sorbitol dehydrogenase (Sdh) 1.1.1.14; L; TC 6.4. Superoxide dismutase (Sod-1, Sod-

2) 1.15.1.1; L; TC 6.4.
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