miR-203 inhibits proliferation and self-renewal of leukemia stem cells by targeting survivin and Bmi-1
Yi Zhang1,2*,7, Shu-yan Zhou3*, Hai-zhao Yan1,Dan-dan Xu1, Hai-xuan Chen4, Xiao-yan Wang2, Xiao Wang2, Yu-ting Liu1, Li Zhang1, Sheng Wang1, Peng-jun Zhou5, Wu-yu Fu5, Bi-bo Ruan5,Dong-lei Ma1, Ying Wang1, Qiu-ying Liu2, Zhe Ren2, Zhong Liu2, Rong Zhang6§, Yi-fei Wang1,2§
1College of life science and technology, Jinan University, Guangzhou, 510632, P.R. China
2Institute of Biomedicine, Jinan University, Guangzhou, 510632, P.R. China
3Department of Pathological Physiology, Wan-nan Medical College,Wuhu, 241000,
P.R. China
4College of medicine, Jinan University, Guangzhou, 510632, P.R. China
5Guangdong Pharmaceutical University
6Department of Endoscopy, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China,Collaborative Innovation Center for Cancer Medicine,
Guangzhou, 510632, P.R. China
7Section of Otolaryngology, Department of Surgery, Yale School of Medicine
*These authors contributed equally to this work
§Corresponding author
Email Address:
Yi Zhang: scholarzy@163.com
Shu-yan Zhou: zhoushuyan_xju2005@126.com
Hai-zhao Yan: yanhaizhao@126.com
Dan-danXu: Junxu98@163.com
Hai-xuan Chen: chx00520@163.com
Xiao-yan Wang: wangxiaoyan_1016@163.com
Xiao Wang: wangxiao0719@163.com
Yu-ting Liu: ziziniu@163.com
Li Zhang: 15692002254@163.com
Sheng Wang: 18520215232@163.com
Peng-jun Zhou: zhouzhang1990@163.com
Wu-yu Fu: fuwuyu2014@163.com
Bi-boRuan: 15011931237@163.com
Dong-lei Ma: donglei131001@163.com
Ying Wang: wy1005swau@aliyun.com
Qiu-ying Liu: qiuying_liu@126.com
ZheRen: rz62@163.com
Zhong Liu: tliuzh@jnu.edu.cn
Rong Zhang: zhangrong@sysucc.org.cn
Yi-fei Wang: twang-yf@163.com
(0086-021-85220504)
Table S1. Demographics and clinical characteristics of 50 newly diagnosed AML patients in this study
Characteristics (n)
|
miR-203
CD34+ expression, n (%) CD34- expression, n (%)
|
Sex
|
|
Female (29)
|
18 (62%) 11 (38%)
|
Male (21)
|
7 (33%) 14 (67%)
|
Pvalue
|
0.157
|
Age
|
|
≤40 years old (37)
|
23 (62%) 14 (38%)
|
>40 years old (13)
|
6 (46%) 7 (54%)
|
Pvalue
|
0.165
|
FAB
|
|
M0 (7)
|
3 (43%) 4 (57%)
|
M1 (10)
|
2 (20%) 8 (80%)
|
M2 (11)
|
4 (36%) 7 (64%)
|
M5 (13)
|
7 (54%) 6 (46%)
|
M7 (9)
|
6 (67%) 3 (33%)
|
Pvalue
|
0.212
|
Laboratory analysis (median)
|
|
WBC (109/L)
|
24.3 17.2
|
Platelet (109/L)
|
5648
|
Hemoglobin (g/dL)
|
12.89.3
|
BM blast, %
|
4532
|
PB blast, %
|
23 14
|
Pvalue
|
0.189
|
Abbreviations: FAB, French-American-Britain subtype; WBC, white blood cell; LDH, lactate dehydrogenase; BM, bone marrow; PB, peripheral blood. *Fisher’s exact test was used.
Table S2. Primers for qRT-PCR
-
Construct Primer sequences (5’-3’)
|
Survivin
Bmi-1
FLT3
WT1
ABCG2
GAPDH
|
F: CACCGCATCTCTACATTCAAGA
R: AAGTCTGGCTCGTTCTCAGTG
F: GGCTCTAATGAAGATAGAGGAG
R: TCACAGTCATTGCTGCTGGGCA
F: GATAGTCACTGAATTCGGTCTTGGA
R: GACATTCTCTGCCACAGAAAACAGC
F: TTACTGGGTGAGGAAATCCA
R: AGATCAACACCCAGTGATGC
F: TCAATCAAAGTGCTTCTTTTTTATG
R: TTGTGGAAGAATCACGTGGC
F: AACGGATTTGGTCGTATTGGG
R: TCGCTCCTGGAAGATGGTGAT
|
Supplemental Figure Legends
Supplemental Fig.1 qRT-PCR of miR-203 expression levels
miR-203 levels were detected after transfection a miR-203 mimic 48h in LSC-enriched
KG-1a and MOLM-13 cell lines and referenced against RNU6B.
| |