Locus nm 199055 1369 bp mrna linear rod 14-oct-2005

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STR M32
LOCUS NM_199055 1369 bp mRNA linear ROD 14-OCT-2005

DEFINITION Mus musculus carbohydrate (N-acetylgalactosamine 4-0)

sulfotransferase 9 (Chst9), mRNA.

ACCESSION NM_199055 XM_128967

VERSION NM_199055.1 GI:39979613

KEYWORDS .

SOURCE Mus musculus (house mouse)

ORGANISM Mus musculus

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;

Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia;

Sciurognathi; Muroidea; Muridae; Murinae; Mus.

REFERENCE 1

AUTHORS Okuda,T., Sawada,T., Nakano,H., Matsubara,K., Matsuda,Y., Fukuta,M.

and Habuchi,O.

TITLE Mouse N-acetylgalactosamine 4-sulfotransferases-1 and -2. Molecular

cloning, expression, chromosomal mapping and detection of their

activity with GalNAcbeta1-4GlcNAcbeta1-octyl

JOURNAL J. Biochem. 134 (1), 111-120 (2003)

PUBMED 12944377

REFERENCE 2 (bases 1 to 1369)

AUTHORS Wheeler,D.L., Church,D.M., Lash,A.E., Leipe,D.D., Madden,T.L.,

Pontius,J.U., Schuler,G.D., Schriml,L.M., Tatusova,T.A., Wagner,L.

and Rapp,B.A.

TITLE Database resources of the National Center for Biotechnology

Information

JOURNAL Nucleic Acids Res. 29 (1), 11-16 (2001)

PUBMED 11125038

COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final

NCBI review. The reference sequence was derived from AB106879.1.

On Jan 1, 2004 this sequence version replaced gi:38083798.

FEATURES Location/Qualifiers

source 1..1369

/organism="Mus musculus"

/mol_type="mRNA"

/db_xref="taxon:10090"

/chromosome="18"

/map="18A2 distal-B1 proximal"

gene 1..1369

/gene="Chst9"

/note="synonyms: MGC130338, 5430438D01Rik"

/db_xref="GeneID:71367"

/db_xref="MGI:1918617"

CDS 40..1281

/gene="Chst9"

/function="A sulfotransferase that transfers sulfate to

position 4 of the nonreducing terminal GalNAc residue."

/note="GalNAc4ST-2;

go_component: Golgi stack [goid 0005795] [evidence IEA];

go_component: integral to membrane [goid 0016021]

[evidence IEA];

go_function: transferase activity [goid 0016740] [evidence

IEA];

go_process: carbohydrate metabolism [goid 0005975]

[evidence IEA]"

/codon_start=1

/product="carbohydrate (N-acetylgalactosamine 4-0)

sulfotransferase 9"

/protein_id="NP_951010.1"

/db_xref="GI:39979614"

/db_xref="GeneID:71367"

/db_xref="MGI:1918617"

/translation="MKAKQVFFSVLLFGTAGLLLFMYLQAWIEEHHTGKIEKKRDQKG

VSVTTGKIQKQITNQNSEVHMPEDLKKKGGDLLNLGSPTRVLRKISHSQRENGAYRST

EAHQGAKIEVFQKPIQMDWPLVTQPLNKSLVQGNKWKKADATQEKRRSFLHEFCKKYG

RVNDPKFNLFHIVSRIYVEDKHKILYCEVPKAGCSNWKRILMVLNGLASSAYNISHDT

VHYGKHLKTLDSFDLKGVHMRLNTYTKAVFVRDPMERLVSAFRDKFEHPNSYYHPVFG

KAIIKKYRPNASAEALNNGSGVKFKEFAYYLLDAHRPVGMDIHWERVSKLCYPCLINY

DFVGKFETLGEDANYFLQLIGAPKELTFPNFKDRHSSDERTNAHVVRQYLKDLSTAER

QLIYDFYHLDYLMFNYTTPHL"

ORIGIN

1 attcagacat gaagagagac acagtggtct gaagtggtca tgaaagccaa acaagtcttc

61 ttttctgtcc tgctgtttgg gacagcaggg cttctgctct tcatgtactt gcaagcatgg

121 attgaagaac atcatacagg gaaaatagag aagaaaaggg atcagaaagg agtatcggtg

181 actacgggaa aaatccagaa acagatcacg aatcagaact ctgaggttca catgcctgaa

241 gatctgaaga agaaaggggg agatctgctc aacctaggga gtccaacaag ggttttaagg

301 aagatcagcc attcacaaag ggagaacgga gcttacagat caactgaagc acatcaagga

361 gctaaaattg aagtttttca gaaacccatc cagatggact ggccactggt cactcagccc

421 ttaaacaaaa gtttggtcca aggcaacaaa tggaagaaag cagatgcaac ccaagagaag

481 cgtcggtcat tccttcatga gttttgcaag aaatatggta gagtaaatga tcccaagttc

541 aacctttttc atatagtatc taggatatat gtagaagaca aacacaaaat cctgtactgt

601 gaagtaccaa aagctggctg ctctaattgg aaaagaattc tgatggtcct aaatggattg

661 gcttcctctg catacaatat ctcccatgat actgtgcact atgggaagca tctgaaaaca

721 ctggatagtt ttgacttaaa aggagtacac atgcgtttga atacatatac caaagctgtg

781 tttgttagag atcccatgga aagattagtc tccgcattta gggataaatt tgagcatccc

841 aatagttact accatccggt gtttggaaag gcaattatca agaaatatcg accaaatgcc

901 tctgcagaag cattaaataa tggatctgga gtcaaattca aagaattcgc ctactatttg

961 ctggatgctc accgtccagt aggaatggat attcactggg aaagagtcag caaactgtgt

1021 tatccgtgtt tgatcaacta tgactttgta gggaagtttg agaccttagg agaggatgcc

1081 aattactttc tacagttgat tggtgctcca aaagagttga catttccaaa ctttaaggat

1141 aggcactcct ctgatgaaag aaccaatgcc cacgtggtaa ggcagtattt aaaggacctg

1201 agcacagccg aaagacagct catctatgac ttctatcact tggactattt gatgtttaat

1261 tacacaactc cacatttgta atttgcattc atttttctaa agccctacat agatttaatg

1321 atgatggcct caaataagct actgtaattg tcctacaatt ctctgtatg
Coding Region
1 atgaaagcca aacaagtctt cttttctgtc ctgctgtttg ggacagcagg gcttctgctc

61 ttcatgtact tgcaagcatg gattgaagaa catcatacag ggaaaataga gaagaaaagg

121 gatcagaaag gagtatcggt gactacggga aaaatccaga aacagatcac gaatcagaac

181 tctgaggttc acatgcctga agatctgaag aagaaagggg gagatctgct caacctaggg

241 agtccaacaa gggttttaag gaagatcagc cattcacaaa gggagaacgg agcttacaga

301 tcaactgaag cacatcaagg agctaaaatt gaagtttttc agaaacccat ccagatggac

361 tggccactgg tcactcagcc cttaaacaaa agtttggtcc aaggcaacaa atggaagaaa

421 gcagatgcaa cccaagagaa gcgtcggtca ttccttcatg agttttgcaa gaaatatggt

481 agagtaaatg atcccaagtt caaccttttt catatagtat ctaggatata tgtagaagac

541 aaacacaaaa tcctgtactg tgaagtacca aaagctggct gctctaattg gaaaagaatt

601 ctgatggtcc taaatggatt ggcttcctct gcatacaata tctcccatga tactgtgcac

661 tatgggaagc atctgaaaac actggatagt tttgacttaa aaggagtaca catgcgtttg

721 aatacatata ccaaagctgt gtttgttaga gatcccatgg aaagattagt ctccgcattt

781 agggataaat ttgagcatcc caatagttac taccatccgg tgtttggaaa ggcaattatc

841 aagaaatatc gaccaaatgc ctctgcagaa gcattaaata atggatctgg agtcaaattc

901 aaagaattcg cctactattt gctggatgct caccgtccag taggaatgga tattcactgg

961 gaaagagtca gcaaactgtg ttatccgtgt ttgatcaact atgactttgt agggaagttt

1021 gagaccttag gagaggatgc caattacttt ctacagttga ttggtgctcc aaaagagttg

1081 acatttccaa actttaagga taggcactcc tctgatgaaa gaaccaatgc ccacgtggta

1141 aggcagtatt taaaggacct gagcacagcc gaaagacagc tcatctatga cttctatcac

1201 ttggactatt tgatgtttaa ttacacaact ccacatttgt aa
NCBI Genome Blast Search
ref|NT_039674.5|Mm18_39714_35 Mus musculus chromosome 18 genomic 2054 0.0

Bottom of Form

Alignments

>ref|NT_039674.5|Mm18_39714_35

Mus musculus chromosome 18 genomic contig, strain C57BL/6J

Length=73768173

Score = 2054 bits (1068), Expect = 0.0

Identities = 1068/1068 (100%), Gaps = 0/1068 (0%)

Strand=Plus/Minus
Query 175 CAGAACTCTGAGGTTCACATGCCTGAAGATCTGAAGAAGAAAGGGGGAGATCTGCTCAAC 234

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12802518 CAGAACTCTGAGGTTCACATGCCTGAAGATCTGAAGAAGAAAGGGGGAGATCTGCTCAAC 12802459
Query 235 CTAGGGAGTCCAACAAGGGTTTTAAGGAAGATCAGCCATTCACAAAGGGAGAACGGAGCT 294

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12802458 CTAGGGAGTCCAACAAGGGTTTTAAGGAAGATCAGCCATTCACAAAGGGAGAACGGAGCT 12802399
Query 295 TACAGATCAACTGAAGCACATCAAGGAGCTAAAATTGAAGTTTTTCAGAAACCCATCCAG 354

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12802398 TACAGATCAACTGAAGCACATCAAGGAGCTAAAATTGAAGTTTTTCAGAAACCCATCCAG 12802339
Query 355 ATGGACTGGCCACTGGTCACTCAGCCCTTAAACAAAAGTTTGGTCCAAGGCAACAAATGG 414

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12802338 ATGGACTGGCCACTGGTCACTCAGCCCTTAAACAAAAGTTTGGTCCAAGGCAACAAATGG 12802279
Query 415 AAGAAAGCAGATGCAACCCAAGAGAAGCGTCGGTCATTCCTTCATGAGTTTTGCAAGAAA 474

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12802278 AAGAAAGCAGATGCAACCCAAGAGAAGCGTCGGTCATTCCTTCATGAGTTTTGCAAGAAA 12802219
Query 475 TATGGTAGAGTAAATGATCCCAAGTTCAACCTTTTTCATATAGTATCTAGGATATATGTA 534

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12802218 TATGGTAGAGTAAATGATCCCAAGTTCAACCTTTTTCATATAGTATCTAGGATATATGTA 12802159
Query 535 GAAGACAAACACAAAATCCTGTACTGTGAAGTACCAAAAGCTGGCTGCTCTAATTGGAAA 594

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12802158 GAAGACAAACACAAAATCCTGTACTGTGAAGTACCAAAAGCTGGCTGCTCTAATTGGAAA 12802099
Query 595 AGAATTCTGATGGTCCTAAATGGATTGGCTTCCTCTGCATACAATATCTCCCATGATACT 654

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12802098 AGAATTCTGATGGTCCTAAATGGATTGGCTTCCTCTGCATACAATATCTCCCATGATACT 12802039
Query 655 GTGCACTATGGGAAGCATCTGAAAACACTGGATAGTTTTGACTTAAAAGGAGTACACATG 714

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12802038 GTGCACTATGGGAAGCATCTGAAAACACTGGATAGTTTTGACTTAAAAGGAGTACACATG 12801979
Query 715 CGTTTGAATACATATACCAAAGCTGTGTTTGTTAGAGATCCCATGGAAAGATTAGTCTCC 774

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12801978 CGTTTGAATACATATACCAAAGCTGTGTTTGTTAGAGATCCCATGGAAAGATTAGTCTCC 12801919
Query 775 GCATTTAGGGATAAATTTGAGCATCCCAATAGTTACTACCATCCGGTGTTTGGAAAGGCA 834

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12801918 GCATTTAGGGATAAATTTGAGCATCCCAATAGTTACTACCATCCGGTGTTTGGAAAGGCA 12801859
Query 835 ATTATCAAGAAATATCGACCAAATGCCTCTGCAGAAGCATTAAATAATGGATCTGGAGTC 894

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12801858 ATTATCAAGAAATATCGACCAAATGCCTCTGCAGAAGCATTAAATAATGGATCTGGAGTC 12801799
Query 895 AAATTCAAAGAATTCGCCTACTATTTGCTGGATGCTCACCGTCCAGTAGGAATGGATATT 954

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12801798 AAATTCAAAGAATTCGCCTACTATTTGCTGGATGCTCACCGTCCAGTAGGAATGGATATT 12801739
Query 955 CACTGGGAAAGAGTCAGCAAACTGTGTTATCCGTGTTTGATCAACTATGACTTTGTAGGG 1014

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12801738 CACTGGGAAAGAGTCAGCAAACTGTGTTATCCGTGTTTGATCAACTATGACTTTGTAGGG 12801679
Query 1015 AAGTTTGAGACCTTAGGAGAGGATGCCAATTACTTTCTACAGTTGATTGGTGCTCCAAAA 1074

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12801678 AAGTTTGAGACCTTAGGAGAGGATGCCAATTACTTTCTACAGTTGATTGGTGCTCCAAAA 12801619
Query 1075 GAGTTGACATTTCCAAACTTTAAGGATAGGCACTCCTCTGATGAAAGAACCAATGCCCAC 1134

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12801618 GAGTTGACATTTCCAAACTTTAAGGATAGGCACTCCTCTGATGAAAGAACCAATGCCCAC 12801559
Query 1135 GTGGTAAGGCAGTATTTAAAGGACCTGAGCACAGCCGAAAGACAGCTCATCTATGACTTC 1194

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12801558 GTGGTAAGGCAGTATTTAAAGGACCTGAGCACAGCCGAAAGACAGCTCATCTATGACTTC 12801499
Query 1195 TATCACTTGGACTATTTGATGTTTAATTACACAACTCCACATTTGTAA 1242

||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 12801498 TATCACTTGGACTATTTGATGTTTAATTACACAACTCCACATTTGTAA 12801451

Score = 194 bits (101), Expect = 7e-47

Identities = 101/101 (100%), Gaps = 0/101 (0%)

Strand=Plus/Minus

Query 1 ATGAAAGCCAAACAAGTCTTCTTTTCTGTCCTGCTGTTTGGGACAGCAGGGCTTCTGCTC 60

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

Sbjct 13065421 ATGAAAGCCAAACAAGTCTTCTTTTCTGTCCTGCTGTTTGGGACAGCAGGGCTTCTGCTC 13065362
Query 61 TTCATGTACTTGCAAGCATGGATTGAAGAACATCATACAGG 101

|||||||||||||||||||||||||||||||||||||||||

Sbjct 13065361 TTCATGTACTTGCAAGCATGGATTGAAGAACATCATACAGG 13065321

Score = 75.7 bits (39), Expect = 5e-11

Identities = 39/39 (100%), Gaps = 0/39 (0%)

Strand=Plus/Minus

Query 139 GTGACTACGGGAAAAATCCAGAAACAGATCACGAATCAG 177

|||||||||||||||||||||||||||||||||||||||

Sbjct 12843279 GTGACTACGGGAAAAATCCAGAAACAGATCACGAATCAG 12843241
Sequence for Primer Design
175-1242
cagaac