SEROLOGY QC
All new reagents lots will be tested using samples and/or commercially available reference material (where available) before being placed in service.
All Serology tests require QC run in parallel with each clinical sample test run. Refer to the appropriate tests for procedure and method.
Record all QC results into the LIS.
Inform the charge technologist or senior technologist of all out-of-range results for the appropriate corrective action. The lab will be notified through LIS e-mail if needed.
Record all corrective action into the LIS.
Inform all out-of range results to the Charge Technologist, Senior Technologist or Quality Assurance Technologist.
Charge technologists are responsible for reviewing overdue Quality Control procedures weekly with responsible bench technologists.
Obtain lists of overdue QC procedures from the LIS - micqc program:
From micqc main menu
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go to 1-Tasks
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select “Results Verification”
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select from pick list “Bacteriology QC Pending List”, “QC Pending List”, “Virology QC Pending List” or “VITEK Bench QC Pending List”.
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F12
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F6 to print list
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Select printer from printer list
Charge technologist, Senior Technologist or Quality Assurance Technologist will verify all Quality Control results weekly. All procedures will be verified on-line.
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Obtain lists for verification from the LIS - micqc program:
From micqc main menu
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go to 1-Tasks
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select “Results Verification”
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select from pick list “Bacteriology Verification List”, “Heating Block Verification List” or “Virology Verification List”.
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Press "enter"
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F12
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Press F6 on the procedure line
If there are abnormal results, follow Steps 7 and 8. If there is no abnormal results continue to Step 9
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F7 to review action if there are abnormal results
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F5 to add new comment/action if needed
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F8 to verify all
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F12
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"y" to confirm editing
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Repeat steps 6 to 11 for further procedures.
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F1 to exit when done.
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For Serology tests, QC verifications are done on worksheet for each assay of each run and documented in the LIS (see LIS Manual section for procedure).
The Laboratory Supervisor will perform Monthly review of all Quality Control procedures.
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Print the list of abnormal results from LIS-micqc:
From micqc main menu
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go to 1-Tasks
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select “Abnormal Res. Rep.”
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select from pick list “monthly Abnormal Log”
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F12
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F7 for report printing
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Select printer from printer list
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Sign and file this report in the QC Review folder.
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Review on-line all QC procedures from LIS-micqc:
From micqc main menu
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go to 1-Tasks
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select “Results Verification”
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F2
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select from pick list “Supervisor Monthly Verification List”
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F12
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F7 for report
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Select "View" from printer list. Wait for 1 minute.
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Scroll down list to review
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"M" then use down arrow to mark a few lines.
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"P" to print the marked page
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Select printer from printer list
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Sign the printed page and file it in the QC Review folder.
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For Serology procedures QC:
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Log on to qc
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1. Result
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Result Maintenance
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Display
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F2 to list instruments, arrow down to select: AXSYM; INSTB (HTLV); MON; VD or VZ
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Enter to select (asterisk)
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F12
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F2 to list tests (8CMS, 8HAB….8VD, 8VZ)
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Enter to select (asterisk)
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F12
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F2 to display QC lots (also displays expiratory dates)
To print or to display more information such as QC verification:
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Enter to select (asterisk)
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F12 to pick date range
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F12, F7 to print
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Sign this report and file it in the QC Review folder.
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Planting
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Blood Cultures
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Genitals
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C.difficile
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Gram Stain
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Urine
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Vitek
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Wounds
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Daily
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Cabinet pressure
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Catalase x 2
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Catalase
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Kit Control (M-F)
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Gram
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Catalase
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Reader temp
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Catalase x 4
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Isoplator
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Oxidase x 2
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Oxidase
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Kohler
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Oxidase
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Change tape
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Oxidase x 4
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N2 tank pressure
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Staph agglu.x 2
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Staph agglu.
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Staph agglu.
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QUAD Screen
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Staph agglu.x 4
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Gram stain slide
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BacT/Alert temp
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Heating Block
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Isoplater Reading X2
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Oxacillin Screen
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Tube coagulase
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WASP x2
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BacT/Alert tape
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Heating Block
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Germ tube
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Cabinet pressure
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Heating Block
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Tube coag
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TDNase
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Gram stain
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Heating Blocks
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Weekly
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Isoplator
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Cefinase
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Sensi cards QC
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WASP X2
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KB QC
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When use
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Eosinophil stain
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Dispenser calibration
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ZN
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Monthly
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WASP X2
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On receipt
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ID and sensi cards
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(on receipt)
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Every 6 months
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Other duties
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Respiratory
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Infection Control
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Mycology
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QC
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Technician
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Serology
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Virology
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Daily
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Catalase x 2
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Catalase x 3
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Calcofluor (M-F)
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s/c CO2 Controls
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Temp. (M-F)
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Cabinet pressure
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Cabinet pressure
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Oxidase x2
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Oxidase
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Cabinet pressure
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(every other day)
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Temp. check
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Staph agglu. x 2
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Staph agglu. x 3
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Oxgall
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Heating Blocks
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Bile solubility
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PYR
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Kohler
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ALA
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Cornmeal
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Heating Block
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Weekly
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Cefinase
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Denka
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Tibutyrin
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Prepared media
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CO2 check
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Nitrogen tank level
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MUG/Indole
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TREK
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CO2 check
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Optochin
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NA
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CF
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Bile solubility
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When use
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ALA
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GenProbe
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All test kits
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PCP/batch
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Xylose
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CMV antigenemia/ batch
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LAP
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Monthly
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Equipment PM
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Clean incubator
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Clean incubator
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Subculture QC
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Isolates from freezer
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Antisera QC
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Respiratory
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Infection Control
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Mycology
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QC
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Technician
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Serology
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Virology
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On receipt
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Fungal media
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3% NaCl
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Indole (TSB)
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QUAD
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Media
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Rapid Yeast
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ALA
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KV
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SMAC
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Antisera QC
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LAP
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Spot Indole
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API
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MAC
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Staph agglutination
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BEAA
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MCPOD
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Strep Grouping
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Bile Esculin
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MGP Broth
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TCBS
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Bile Solubility
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ML
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Thermonuclase
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Brilliance VRE
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Motility media
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Thermometers
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Campylobacter
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MR-VP
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Tibutyrin
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CARROT Broth
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MSAOX
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TSI
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Catalase
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Mueller Hinton
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Tube coagulase
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Cefinase
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MUG/Indole
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Urea
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CF
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NA
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VISA
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Choc
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NaCl
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ZN
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Denium Blue
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Novobiocin
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EBM
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OCBL
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Eosinophil
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ONPG
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EV
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Ornithine broth
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GMSL
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Optochin
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Gram stain
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Oxacillin screen
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GT
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Oxidase
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HI
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Phadebact
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IMA
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PYR
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Every 6 months
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Pipette check
(send-out)
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Centrifuge check
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Other duties
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Trouble shoot QC problems
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New product evaluation
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