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Electronic Supplementary Material
Electronic Supplementary Table (ESM) Table 1. Phylogenetically controlled GLS regressions between sperm characteristics and heterozygosity. Three separate analyses were performed using 1) data from all species, 2) data from endangered species only and 3) data from non-endangered species. The strength of the phylogenetic effect was assessed using the scaling parameter  (superscripts indicate if  was significantly different than 0 and 1 respectively, with non significant values indicated by ns and significant (p<0.05) values indicated by *). Effect size, r, degrees of freedom, and the non-central 95% confidence intervals (CI) are presented for each calculation. CIs are considered significant when the range of values do not overlap zero. Significant relationships are presented in bold text.
Electronic Supplementary Table (ESM) Table 2. Heterozygosity (He), sperm characteristics, conservations status and mating system data for the mammals examined in this study. Species conservation status was determined using the IUCN Red List (En = endangered, Non-en = non-endangered). For brevity we only report results from the IUCN Red List throughout this study, but we obtained qualitatively similar results in all analyses when species conservation status was determined using the Convention on International Trade in Endangered Species (CITES). Mating systems were broadly classified as monogamous (M) or polygamous (P). Little is known about the reproductive biology of tigrinas, however as felines are generally polygamous we classified this species as P. Source data are indicated with subscripts.

Trait



Predictor

n

R2adjusted

Slope

t

p

r

df

CI

All Species




























% abnormal sperm

< 0.001ns, *

heterozygosity

20

0.17

-0.64

-2.23

0.04

-0.47

18

-0.71 to -0.03




% motile sperm

< 0.001ns, *

heterozygosity

18

0.25

2.12

2.60

0.02

0.54

16

0.10 to 0.76

Endangered Species

























% abnormal sperm

< 0.001ns, *

heterozygosity

10

0.43

-1.17

-2.80

0.02

-0.70

8

-0.88 to -0.13




% motile sperm

0.75 ns, ns

heterozygosity

10

0.47

2.60

2.98

0.02

0.73

8

0.17 to 0.88

Non-Endangered Species































% abnormal sperm

1.0 ns, ns

heterozygosity

10

-0.12

0.002

0.005

1.0

0.002

8

-0.57 to 0.57




% motile sperm

1.0 ns, ns

heterozygosity

8

-0.13

0.88

0.44

0.68

0.18

6

-0.54 to 0.70
ESM Table 1

ESM Table 2











Heterozygosity

Sperm Characteristics







Species

Latin name

He

Number of Loci

N

% abnormal sperm

% motile sperm

N


Status code

Mating system

Carnivora































Black-footed ferret

Mustela nigripes

0.371,2

5-7

7-78

46.815

58.515

29

En

P2




Black bear

Ursus thibetanus japonicus

0.523,4

6-10

50-67

48.316, 17

47.2516,17

4-18

En

P32




Cheetah

Acinonyx jubatus raineyi

0.466

82

10

81.619

6919

97

En

P34




Giant panda

Ailuropoda melanoleuca

0.748

7

22

14.022

7922

4

En

P33




Serengeti Lions

Panthera leo

0.476

88

10

24.824

9124

8

En

P34




Ngorogoro Lions

Panthera leo

0.406

88

10

50.524

8324

9

En

P34




Asiatic Lions

Panthera leo persica

0.086

88

10

66.224

6124

8

En

P34




Florida panther

Puma concolor coryi

0.156

84

10

93.527

38.327

16

En

P34




Red wolf

Canis rufus

0.727

4

48

26.428

71.228

13

En

M34




Brown bear

Ursus arctos yesoensis

0.275

15

13

21.818

80.218

10

Non-En

P33




Coyote

Canis latrans

0.817

8

10

12.120

86.120

10

Non-En

M34




Domestic cat

Felis catus

0.686

93

10

29.121

7721

16

Non-En

P35




Jaguar

Panthera onca

0.839

12

6

26.523

7323

6

Non-En

P36




Margays

Leopardus wiedii

0.8210

4

25

42.625

73.525

3

Non-En

P37




Ocelot

Leopardus pardalis

0.8410

4

77

17.625

81.425

3

Non-En

P37




Panther

Puma concolor

0.686

84

10

66.526

n/a

35

Non-En

P34




Tigrinas

Leopardus tigrinus

0.8310

4

46

40.825

71.425

4

Non-En

P

Lagomorpha































Rabbit

Oryctolagus cuniculus

0.4611

29

1-29

13.711

n/a

1-29

Non-En

P36

Artiodactyla































Bison

Bison bison

0.6312

15

21-40

26.229

68.829

21

Non-En

P33

Primates































Howler monkey

Alouatta caraya

0.5613,14

9-11

20-48

29.430,31

76.230,31

6-9

Non-En

P30


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