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Curriculum Vitae Ming-Daw Tsai, Ph. D


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Brief Summary of Research Accomplishment
Dr. Tsai’s research has focused on “Biophosphates: from Enzymes to Signaling”. He pioneered a widely adopted approach in which advanced bioorganic chemical methods are integrated with structural biological studies to uncover reaction mechanisms and signaling interactions involving biophosphates. Since biophosphates are central to biology and are involved in innumerable physiological functions, his research has had high impact that spans multiple disciplines of biological science, as highlighted below:
A. Chiral Phosphates, Thiophosphates and Mechanism of Adenylate Kinase. Tsai was among several major players who developed the field of chiral phosphates and thiophosphates. In 1979, he discovered the effect of 17O on 31P NMR, which made it possible to analyze the configuration of chiral [17O,18O] phosphates. He was also the first to develop synthesis and configurational analysis of chiral [16O,17O,18O,S] phosphate. He then used chiral dNTP analogs to resolve important mechanistic problems of muscle adenylate kinase, and to discover complete reversal and enhancement of phosphorus stereoselectivity by single mutations.
B. Phospholipids Chiral at Phosphorus and Mechanism of Phospholipases. Since 1982 Tsai pioneered syntheses and configurational analyses of phospholipids chiral at phosphorus and related phosphatidylinositol analogs, and used them to elucidate the mechanism of phospholipases A2, C, and D, which established his leadership in the field of phospholipase enzymology. Highlights include a 2001 “Chemical Reviews” article on phospholipase A2, and discovery of a novel catalytic triad Arg…Asp…His in phospholipase C.
C. Cyclin-dependent Kinase 4 (CDK4) Inhibitors – Ankyrin-repeat Tumor Suppressors. In 1998 Tsai reported the first structure of a new tumor suppressor p16, an inhibitor of CDK4, and subsequently determined other structures in the family (p15, p18, p19, and gankyrin). These and other studies led to a definitive review on ankyrin repeat proteins. This work contributed to the understanding of how the kinase activity of CDK4 is regulated by p16, work of fundamental relevance to both normal cellular proliferation and cancer.
D. Mechanism of DNA Polymerases. From 1998 Tsai used inert metal-dNTP complexes to resolve reaction intermediates of Pol , then characterized intermediate structures and measured microscopic rates. A major finding is that, in contrast to the then prevailing theory that fidelity is dictated by the rate-limiting, dNTP-induced conformational change, the latter is faster than the chemical step. In a related front, he discovered that Pol X from African swine fever virus is specific to G:G mispair in addition to Watson-Crick base pairs. He then solved the structures of Pol X and Pol X-DNA-MgdNTP ternary complex by NMR, and elucidated the structural and mechanistic basis for how Pol X overcomes Watson-Crick base pairing to achieve G:G mispair. He recently also demonstrated a new approach to design anti-influenza inhibitors by disrupting the trimeric structure of the nucleoprotein, leading to inhibition of the viral RNA polymerase and viral replication.
E. FHA Domain: Phosphothreonine Specificity and Phosphate Counting. Tsai is among the pioneers of structural and functional studies of the pThr-specific FHA domain. In 1999 Tsai reported the first structure of an FHA domain and subsequently established his leadership in this field with two important structures of protein-phosphoprotein complexes - human Ki67 FHA complexed with a phosphorylated domain of NIFK, and yeast Dun1 FHA complexed with the dual-phosphorylated SCD domain of Rad53. The latter led to discovery of a novel “phosphate counting mechanism” in signaling. In 2014 he further used quantitative MS analysis to elucidate the mechanisms of phospho-priming and auto-activation of Rad53 in vivo. Currently Tsai’s lab is actively investigating the biological functions and mechanisms of FHA domains from several human proteins in DNA damage response and cancer signaling, including CHK2, MDC1, and TIFA.
F. Histone Demethylases. In 2006, several labs discovered JmjC histone demethylases. In work extraneous to his studies of biophosphates, Tsai independently used global analyses of histones by mass spec to discover four histone demethylases in yeast. In addition, he determined the structure of the ARID domain of human histone demethylase RBP2 and identified its DNA binding specificity.

Invited Lectures

1. "31P(17O) NMR Studies of Metal-Nucleotide Interactions". Gordon Conference on Enzymes, Coenzymes, and Metabolic Pathways, July 7-11, 1980.

2. "Biochemical Application of NMR Methods Involving Oxygen Isotopes". Purdue University Biochemistry Program, January 18, 1982.

3. "Biochemical Application of 17O NMR and 31P(17O) NMR". Federation Meeting, Minisymposium on Spectroscopic Applications of 17O in Biological Chemistry, April 20, 1982.

4. "Phospholipids Chiral at Phosphorus, Stereochemistry of Reactions Catalyzed by Phospholipases". University of Chicago, Biophysics and Theoretical Biology, March 11, 1983.

5. "NMR, Chirality, Enzymes, and Membranes". Gordon Conference on Enzymes, Coenzymes, and Metabolic Pathways," July 4, 1983.

6. "Phospholipids Chiral at Phosphorus. Use of Chiral Thiophosphatidylcholine to Study the Metal-Binding Properties of Bee Venom Phospholipase A2". Fourth Midwest Enzyme Conference, Chicago, IL, October 27, 1984.

7. "Synthesis and Biochemical Properties of Phospholipids Chiral at Phosphorus". Brown University, Dept. of Chemistry, March 1, 1985.

8. "17O NMR Study on the Interaction of Adenine Nucleotides with Metal Ions and with Myokinase". ACS 189th National Meeting, Symposium on Biological NMR, Miami Beach, May 1, 1985.

9. "Phospholipids Chiral at Phosphorus. Use of Chiral Thiophospholipids to Study the Mechanism of Phospholipase A2". Steenbock Symposium on Stereochemistry of Enzymatic Reactions, University of Wisconsin, Madison, July 1-3, 1985.

10. "Use of 17O NMR and 31P NMR to Study Metal-Nucleotide-Enzyme Interactions". University of Maryland, Dept. of Chemistry, November 5, 1985

11. "Phospholipids Chiral at Phosphorus". Gordon Conference on "Enzymes, Coenzymes, and Metabolic Pathways", June 29 - July 4, 1986

12. "Phospholipids Chiral at Phosphorus. Stereochemistry of Enzymatic Reactions" Tenth International Conference on Phosphorus Chemistry, Bonn, W. Germany, August 31 - September 6, 1986.

13. "Phospholipids Chiral at Phosphorus". Second International Symposium on Phosphorus Chemistry Directed Toward Biology, Poland, September 7-12, 1986.

14. "Phosphorothioates: Preparation and Utilization of Phosphate Analogs". University of Texas, Health Science Center at San Antonio, Department of Biochemistry, Feb. 26, 1987.

15. "Biochemical and Biophysical Properties of Phospholipids Chiral at Phosphorus". University of Texas, Health Science Center at San Antonio, Department of Biochemistry, February 27, 1987.

16. "Phospholipids Chiral at Phosphorus". University of Kentucky, Department of Chemistry, March 13, 1987.

17. "Phospholipids Chiral at Phosphorus". University of Illinois, College of Medicine at Chicago, Department of Biological Chemistry, May 7, 1987.

18. "Phospholipids Chiral at Phosphorus". ACS 21st Middle Atlantic Regional Meeting, Pomona, NJ, May 20, 1987.

19. "Phospholipids Chiral at Phosphorus". University of Akron, Department of Chemistry, September 15, 1987.

20. "Gene Synthesis and Protein Engineering". OSU Industrial/Academic Chemistry Program, October 10, 1987.

21. "Is There Interfacial Activation in the Catalysis of Phospholipase A2?" Seventh Midwest Enzyme Conference, University of Chicago, October 17, 1987.

22. "Phospholipids Chiral at Phosphorus". Second SCBA International Symposium and Workshop, Symposium on Bioorganic and National Product Chemistry,Berkeley, June 27-30, 1988.

23. "Stereochemistry of Phospholipases". Smith and Kline and French Laboratories, November 17, 1988.

24. "Use of Site-Directed Mutagenesis to Study the Structure-Function Relationship of Adenylate Kinase". Ohio State Biochemistry Program, February 21, 1989.

25. "Bioorganic Chemistry: From Small Molecules to Macromolecules". University of Washington, Seattle, Department of Chemistry, March 10, 1989.

26. "Stereochemistry and Mechanism of Phospholipases". Boston University, Department of Chemistry, March 20, 1989.

27. "Enzyme Mechanisms: From Substrate Engineering to Protein Engineering". Dept. of Biochemistry and Molecular Biology, Univ. of Chicago, May 10, 1989.

28. "Mechanism of Adenylate Kinase". Dept. of Biophysics, Max-Planck Institute for Medical Research, Heidelberg, West Germany, June 14, 1989.

29. "Mechanism of Adenylate Kinase". Institute of Organic and Biochemistry, University of Freiburg, Freiburg, West Germany, June 15, 1989.

30. "Bioorganic Chemistry: From Conventional to Contemporary". Institute of Chemistry, Academia Sinica, Taipei, Taiwan, Sept 4, 1989.

31. "Bioorganic Chemistry: From Conventional to Contemporary". Institute of Enzyme Research, University of Wisconsin at Madison, Sept. 14, 1989.

32. "Mechanism of Adenylate Kinase". Department of Biochemistry and Biophysics, Texas A&M University, November 1, 1989.

33. "Structural and Functional Studies on the Mechanism of Adenylate Kinase". Department of Chemistry, New Mexico State University, March 8, 1990.

34. "Gene Synthesis, Expression, and Protein Engineering of Phospholipase A2 from Bovine Pancreas". Department of Biochemistry and Molecular Biology, New Mexico State University, March 9, 1990.

35. "Perfecting an Enzyme: A phospholipase A2 with Significantly Improved Catalytic Activity". American Chemical Society National Meeting, Boston, Apr. 22-27, 1990.

36. "Enzyme Mechanisms: From Substrate Engineering to Protein Engineering". Third SCBA International Symposium and Workshop, Hong Kong, June 26-30, 1990.

37. "Protein Engineering of Phospholipase A2 From Bovine Pancreas". Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, July 2, 1990.

38. "A Chemist's Approach to Biomedical Research". Veterans General Hospital, Taipei, Taiwan, July 3, 1990.

39. "New Approaches in Bioorganic Chemistry". Department of Chemistry, National Taiwan University, Taipei, July 9, 1990.

40. "Structure-Function Studies of Large Organic Molecules-Enzymes". International Symposium for Chinese Organic Chemists, Shanghai, July 12-14, 1990.

41. "Mechanism of Adenylate Kinase: Have Previous NMR and X-Ray Results Passed the Test by Site-Directed Mutagenesis?" Monsanto Company, St. Louis, August 7, 1990.

42. "Modern Bioorganic Chemistry: Structure-Function Studies of Enzymes". Department of Chemistry, St. Olaf College, Northfield, Minnesota, Setp. 14, 1990.

43. "Mechanism of Adenylate Kinase: Site-Directed Mutagenesis Versus X-Ray and NMR", Kent State University, Kent, Ohio, October 4, 1990.

44. "Mechanism of Adenylate Kinase: Have Previous NMR and X-Ray Results Passed the Test by Site-Directed Mutagenesis?" Department of Chemistry, University of Delaware, November 5, 1990.

45. "Mechanism of Adenylate Kinase: Site-Directed Mutagenesis Versus X-Ray and NMR". Department of Chemistry, Washington State University, Pullman, WA, Feb. 19, 1991.

46. "Protein Engineering of Phospholipase A2". Department of Chemistry, California Institute of Technology, Pasadena, CA, Feb. 27, 1991. (Joint Organic Chem./Chem. Biology)

47. "Protein Engineering of Phospholipase A2". School of Pharmacy, University of Cincinnati, Cincinnati, Ohio, April 4, 1991.

48. "Protein Engineering of Bovine Pancreatic Phospholipase A2". Royal Netherlands Academy of Arts and Sciences, Colloquium on Molecular Biology and Inhibition of Cellular and Extracellular Phospholipases A2, Amsterdam, April 23-27, 1991.

49. "Mechanism of Adenylate Kinase: Manipulating Phosphorus Stereochemistry by Site-Directed Mutagenesis". Regional Meeting of the American Chemical Society, Indianapolis, May 29-31, 1991.

50. "Phospholipase A2 Engineering". National Meeting of the American Chemical Society, New York City, Aug. 25-30, 1991.

51. "Structure-Function Relationship of Adenylate Kinase", Fox Chase Institute for Cancer Research, Philadelphia, September 19, 1991.

52. "Manipulating the Phosphorus Stereospecificity of an Enzyme", Dept. of Chemistry, University of Chicago, Nov. 15, 1991.

53. "Iterative Structure-Function Studies of Enzymes: A Case in Adenylate Kinase", Institute of Biological Chemistry, Academia Sinica, Taipei, Dec. 27, 1991.

54. "Modern Bioorganic Chemistry: Structure-Function Relationship of Enzymes", Institute of Chemistry, Academia Sinica, Taipei, Dec. 30, 1991.

55. "Structure-Function Relationship of Adenylate Kinase", Dept. of Biology, Syracuse University, March 6, 1992. (host: Richard Levy)

56. "Structure-Function Relationship of Adenylate Kinase", University of Florida College of Medicine, March 26, 1992. (host: David Silverman)

57. "Biochemical Applications on NMR", Dept. of Chemistry, Denison University, Granville, Ohio, April 23, 1992. (host: Richard Doyle)

58. "Structure-Function Relationship of Adenylate Kinase", Wright State University, Dayton Ohio, April 24, 1992. (host: Lawrence Prochaska)

59. "Stereochemical Mechanism of Phospholipase C", FASEB Summer Conference on Phospholipases, Saxtons River, Vermont, July 12-17, 1992. (Organizer: Ed Dennis)

60. "Structure-Function Relationship of Adenylate Kinase", Biotechnology Research Institute, National Research Council of Canada, Montreal, Sept. 2, 1992. (Host: Feng Ni)

61. "Structure-Function Relationship of Adenylate Kinase", Max-Planck Institute, Gottingen, Germany, Setp. 14, 1992. (Host: Fritz Eckstein)

62. "Structure-Function Relationship of Adenylate Kinase", EMBO Workshop, Germany, Sept. 16-19, 1992. (Organizer: Fred Wittinghofer)

63. "Structure-Function Relationship of Phospholipase A2", Dept. of Chemistry, Indiana University, Oct. 23, 1992. (Host: David Daleke)

64. "Structure-Function Relationship Enzymes: a Case Study with Phospholipase A2", Park Davis, Ann Arbor, May 4, 1993. (Host: Don Hupe)

65. "Structure-Function Relationship of Adenylate Kinase", Ann Arbor Enzymes Club, May 5, 1993. (Host: James Coward)

66. "Modern Bioorganic Chemistry: Structure-Function Relationship of Enzymes", Department of Chemistry, Tsing-Hua University, Taiwan, July 12, 1993.

67. "Improving the Structure-Function Relationship of Enzymes", Institute of Chemistry, Academia Sinica, Taipei, July 13, 1993.

68. "Structure-Function Relationship of Phospholipase A2", Dept. of Chemistry, Wayne State University, Sept. 24, 1993. (Host: Shahriar Mobashery)

69. "Mechanism of Adenylate Kinase. 1H, 13C, and 15N NMR Assignments, Secondary Structures, and Substrate Binding Sites", Thirteenth Midwest Enzyme Chemistry Conference, Chicago, October 9, 1993.

70. "NMR Analysis of the Structure of the Adenylate Kinase-MgAP5A Complex", International Symposium on Adenylate Kinase, Yamaguchi University, Japan, March 26-28, 1994.

71. "Adenylate Kinase: From Molecular Biology to NMR Structure", Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, March 28, 1994. (Host: Bai-Ling Lin)

72. "Structure-Function Relationship of Adenylate Kinase", Chicago Medical School, Chicago, May 5, 1994. (Host: Bob Kemp)

73. "Assignment and Secondary Structures of a 22 kDa System at pH 7.1: Adenylate Kinase Complex with MgAP5A", Varian NMR Users Conference, Akron, August 18, 1994. (Host: Peter Rinaldi)

74. "Structure-Function Relationship of Adenylate Kinase", Floss Symposium, Seattle, Washington, August 27, 1994.

75. "Structure-Function Relationship of Adenylate Kinase", School of Pharmaceutical Sciences, Univ. of California at San Francisco, San Francisco, Sept. 15, 1994. (Host: George Kenyon)

76. "Structure-Function Relationship of Phospholipase A2", Department of Medicinal Chemistry, University of Illinois at Chicago, October 3, 1994. (Host: Karol S. Bruzik)

77. "Adenylate Kinase: From Molecular Biology to Total Assignment by NMR", Department of Chemistry, University of Illinois at Chicago, October 4, 1994. (Host: Wan-Hua Cho)

78. "Structure-Function Relationship of Adenylate Kinase", Dept. of Biophysics and Physiology, Case Western Reserve University, October 17, 1994. (Host: C. R. Sanders)

79. "Syntheses and Biochemical Applications of Phosphoinositides", The 4th Tohwa University International Symposium on Chemistry on the Biologically and Physiologically Active Natural Products, Fukuoka, Japan, November 19-22, 1994.

80. "Modern Bioorganic Chemistry: Structure-Function Relationship of Enzymes." Department of Chemistry, Case Western Reserve University, Cleveland, March 9, 1995. (Host: Michael Zagorski)

81. "Structure-Function Relationship of Adenylate Kinase." Department of Chemistry, Miami University, Oxford, Ohio, March 30, 1995. (Host: John F. Sebastian)

82. "Syntheses and Biochemical Applications of Phosphoinositides", Institute of Chemistry, Academia Sinica, Taipei, May 6, 1995.

83. "Structural Analysis of a 22 kDa System at pH 7.1 by NMR: Adenylate Kinase Complex with MgAP5A", Midwest Regional ACS Meeting, Akron, May 31, 1995

84. "Structural Determination of Adenylate Kinase, A 22 kD Protein", Fourteenth American Peptide Symposium, Columbus, June 18-23, 1995.

85. "From Protein Engineering to Drug Design". International SCBA Symposium on Rational Drug Design, Vancouver, June 25-30, 1995.

86. "Adenylate Kinase: A Model or an Exception?", Gordon Conference on Enzymes, Coenzymes, and Metabolic Pathways, New Hampshire, July 16-21, 1995.

87. "Structure-Function Relationship of Phospholipase A2", FASEB Summer Conference on Phospholipases, Vermont, July 22-27, 1995.

88. "Protein Structural Analysis by NMR", Department of Physics, Indiana University-Purdue University at Indianapolis, December 7, 1995.

89. "Design and Construction of a New Restriction Endonuclease Specific to the HIV Genome", International Symposium on Perspectives on Protein Engineering, Le Corum Montpellier, France, March 2-6, 1996.

90. "Structure-Function Relationship of Adenylate Kinase", Department of Chemistry, SUNY Stony Brook, March 25, 1996. (Host: Nicole Sampson)

91. "Modern Bioorganic Chemistry", The Fourth International Symposium for Chinese Organic Chemists, Hong Kong, April 5-8, 1996.

92. "Structure-Function Relationship of DNA Polymerase " EMBO Meeting on Nucleotidyl and Phosphoryl Transfer in the Protein and RNA World, Xanten, Germany, Sept. 29 - Oct. 3, 1996.

93. "Structure-Function Relationship of Tumor Suppressor P16." Cancer Center, Ohio State University, November 6, 1996. (Host: Lee Johnson)

94. "Protein Engineering". Department of Chemical Engineering, Ohio State University, December 5, 1996. (Host: Shang-Tien Yang)

95. "Structural Analysis of Tumor Suppressor P16 by NMR". American Chemical Society Regional Meeting, Midland, Michigan, May 27, 1997.

96. "Adenylate Kinase: Site-directed Mutagenesis Versus NMR and X-ray". Protein Engineering Group, University of Toronto, May 29, 1997. (Host: Robert Reedjik)

97. "Probing Enzyme Mechanisms with Bridging and Nonbridging Sulfur Analogs of Nucleotides and Phospholipids". Department of Chemistry, University of Toronto, May 30, 1997. (Host: Andrew Woolley)

98. "Structure-Function Relationship of Bacterial PI-Phospholipase C." National Meeting of the American Chemical Society, Las Vegas, Sept. 7-11, 1997.

99. "Mechanism of PI-Specific Phospholipase C". Dept of Chemistry, Scripps Research Institute, September 12, 1997. (Host: Chi-Huey Wong)

100. "Structure-Function Relationship of PI-specific Phospholipase C". Dept of Biochemistry, Michigan State University, November 3, 1997. (Host: Honggao Yan).

101. "Mechanism of PI-Specific Phospholipase C". Dept of Pharmacology, Albert Einstein School of Medicine, New York, December 8, 1997. (Host: Zong-Yin Zhang)

102. "Structure-Function Relationship of Adenylate Kinase". University of Texas at Austin, January 23, 1998. (Host: Jon Robertus)

103. "Solution Structure of Tumor Suppressor p16 by NMR". Otterbein University, Westerville, Ohio, April 15, 1998. (Host: Chihae Yang)



  1. "Structure and Function of Tumor Suppressor p16". Dept. of Chemistry, Florida State University, April 28, 1998. (Host: Alan Marshall)

  2. “Structure and Mechanism of Tumor Suppressor p16INK4A”. Department of Biochemistry, Case Western Reserve University, May 6, 1998.

  3. "Use of Thiophosphate Analogs to Probe the Mechanism of PI-Phospholipase C". 14th International Conference on Phosphorus Chemistry, Cincinnati, July 12-17, 1998.

  4. "Structure-Function Analysis of the INK4 Family of Tumor Suppressors". Department of Biochemistry, University of Toledo, October 15, 1998. (Host: James Slama)

  5. “A Novel, Dual-Function Catalytic Triad Arg-Asp-His for P-O Bond Cleavage Catalyzed by PI-PLC”. ASBMB Fall Symposia, Lake Tahoe, CA, Oct 23-26, 1998.

  6. "Structure-Function Analysis of the INK4 Family of Tumor Suppressors", NMR Technologies: Development and Applications Conference, Baltimore, October 29-30, 1998.

  7. “A Novel, Dual-Function Catalytic Triad Arg-Asp-His for P-O Bond Cleavage Catalyzed by PI-PLC”. Dept of Chemistry, National Taiwan University, Nov. 10, 1998.

  8. "Structure-Function Analysis of the INK4 Family of Tumor Suppressors". Dept of Chemistry, Tsinghua University, Nov. 11, 1998.

  9. “A Novel, Dual-Function Catalytic Triad Arg-Asp-His for P-O Bond Cleavage Catalyzed by PI-PLC”. Institute of Chemistry, Academia Sinica, Nov. 13, 1998.

  10. "Structure-Function Analysis of the INK4 Family of Tumor Suppressors". Department of Chemistry, Kent State University, November 19, 1998. (Host: Scott Prosser)

  11. “Identification of a Novel Catalytic Triad Arg-Asp-His from PI-specific Phospholipase C”. Canadian Society of Chemistry National Meeting, May 29-31, 1999.

  12. “Solution Structure of the FHA2 Domain of RAD53”. 31st Central Regional Meeting of the American Chemical Society, Columbus, Ohio, June 21-23, 1999.

  13. “Solution Structures and Functional Analyses of Tumor Suppressors p16 and p18”. SCBA International Symposium, Hong Kong, August 14-19, 1999.

  14. “Structure and Function of a New Phosphoprotein Binding Domain FHA2 from Yeast Rad53.” Midwest Enzyme Chemistry Conference, Chicago, October 2, 1999.

  15. “New Structural Motifs for Protein-Protein Interactions in Cancer-related Pathways.” University of Akron, November 23, 1999. (Host: Matthew Espe)

  16. “Structure, Function, and Specificity of FHA, a New Phosphoprotein Binding Domain”. Keynote Speaker, Volcano Conference, Seattle, Feb. 25-27, 2000.

  17. “Mechanism of PI-Specific Phospholipase C”. FASEB Summer Conference on Phospholipases, Snowmass Village, Colorado, July 8-13, 2000.

  18. “Structure, Function, and Specificity of FHA, a New Phosphoprotein Binding Domain”. Bruker Users Conference, Columbus, Ohio, October 5-6, 2000.

  19. “Structure and Specificity of FHA, a New Phosphoprotein Binding Domain in Signal Transductions”. OSBP Symposium Keynote Speaker, October 7, 2000.

  20. “Structure, Function, and Specificity of FHA, a New Phosphoprotein Binding Domain with Dual Specificity.” Cambridge Healthtech Institute Conference on Protein Structure. McLean, Virginia, October 26-27, 2000.

  21. “Structure and Specificity of FHA, a New Phosphoprotein Binding Domain in Signal Transduction.” Institute of Biophysics, Academia Sinica, Beijing, Oct. 31, 2000.

  22. “Structure and Specificity of FHA, a New Phosphoprotein Binding Domain in Signal Transduction.” Department of Chemistry, Beijing University, Nov. 3, 2000.

  23. “Structure and Function of the INK4 Family of Tumor Suppressors.” Institute of Biophysics, Academia Sinica, Beijing, Nov. 3, 2000.

  24. Structure and Mechanism of Phospholipase A2 from Bovine Pancreas. Institute of Biophysics, Academia Sinica, Beijing, Nov. 7, 2000.

  25. Structure and Specificity of FHA, a New Phosphoprotein Binding Domain in Signal Transduction. Department of Chemistry, Tsinghua University, Nov. 9, 2000.

  26. Structure-Function Relationship of Phosphatidylinositol-specific Phospholipase C. Institute of Biophysics, Academia Sinica, Beijing, Nov. 10, 2000.

  27. “Structure, Function, and Specificity of FHA, a New Phosphoprotein Binding Domain”. Eleventh International Conference on Second Messengers and Phosphoproteins, Melbourne, Australia, April 22-26, 2001.

  28. “Structure, Function, and Specificity of FHA, a New Phosphoprotein Binding Domain”. Bioorganic Chemistry Gordon Conference, June 17-22, 2001.

  29. “A DNA Polymerase with Specificity for Four Correct and One Mismatch Base Pairs”. Wayne State University Dept of Chemistry, Nov. 2, 2001. (Mark Spaller, host)

  30. “Structure, Function, and Specificity of FHA, a New Phosphoprotein Binding Domain”. Department of Pharmacology, Ohio State University, Nov. 13, 2001. (Dale Hoyt, host)

  31. “Chemistry at the Interface of Biology”. Department of Chemistry, Central State University, Dayton, Ohio, Feb. 14, 2002. (Willie Houston, Host)

  32. Symposium on "Modern Aspects of Structure Function Correlation of Biomolecules", National Meeting of the American Chemical Society, Orlando, Florida, April 7-11, 2002.

  33. “A Simplified Model for the Fidelity of DNA Polymerases”. Gordon Research Conference on Mutagenesis, Main, July 28-Aug. 2, 2002.

  34. “Structure and Mechanism of DNA Polymerases”. UTMB Galvaston, TX, September 9, 2002. (James Lee, host)

  35. “Chemical Basis of DNA Polymerase Fidelity – the Way We Survive”. Evans Lecture Event, October 4, 2002.

  36. “A Simplified Mechanism for the Fidelity of DNA Polymerases”. Vanderbilt University, October 11, 2002.

  37. “Structure and Mechanism of High-Fidelity and Low-Fidelity DNA Polymerases”. Institute of Molecular Biology, Academia Sinica, October 21, 2002.

  38. “A New View to the Fidelity Mechanism of High-Fidelity and Low-Fidelity DNA Polymerases”. Michigan State University, Nov. 8, 2002. (Honggao Yan, host)

  39. “Structure and Mechanism of High-Fidelity and Low-Fidelity DNA Polymerases”. Texas A&M University, March 26, 2003. (Paul Fitzpatrick, host)

  40. “Structure and Mechanism of High-Fidelity and Low-Fidelity DNA Polymerases”. Thomas Jefferson University, Phidelphia, May 6, 2003. (Ya-ming Hou, host)

  41. “Opportunities for Chemists in the Post-genomic Era”. CACS-Tristate Chapter Symposium. Schering-Plough Research Institute, June 7, 2003.

  42. “DNA Polymerases:  from Chemistry to Biology", Dept of Chemistry, Tsinghua University, Beijing, Nov. 7, 2003

  43. “DNA Polymerases:  from Chemistry to Biology", Dept of Chemistry, Beijing University, Beijing, Nov. 10, 2003

  44. “DNA Polymerases:  from Chemistry to Biology", Dept of Chemistry, Tsinghua University, Hsinchu, Nov. 18, 2003

  45. “Structure and Mechanism of High-Fidelity and Low-Fidelity DNA Polymerases”. Dept of Chemistry, Georgia State University, December 12, 2003. (Jenny Yang, host)

  46. “Structure and Mechanism of High-Fidelity and Low-Fidelity DNA Polymerases”. Dept of Biochemistry, Duke University, Jan 23, 2004. (Johannes Rudolph, host)

  47. “Structural Basis of the Ligand Specificity of FHA Domains”, Biophysics Program, Institute of Physics, Academia Sinica, Taipei, March 29, 2004. (Tsong Tian Yow, host)

  48. “FHA Domains:  from Chemistry to Biology", College of Life Sciences, Chiao-Tung University, Hsinchu, March 30, 2004. (Yuh-Shyong Yang, host)

  49. “Enzymatic Evidence for a Low-Fidelity Base Excision Repair Pathway Encoded by African Swine Fever Virus”, Research Division, Veterans Hospital, Taipei, April 2, 2004. (Ming-Shi Hsiao, host)

  50. “Structural Basis of the Ligand Specificity of the FHA Domain of Human Tumor Suppressor Chk2”, Symposium on “Drug Discovery by Chemical Genomics Approach”, 19th Joint Biological Societies Conference (JBSC), Taipei, April 10-11, 2004. (Also served as session chair.)

  51. “NMR structures of proteins related to DNA damage and cancer”. Structural Biology Group, NIH, May 10, 2004. (Host: Angela Gronenbon)

  52. “Structure and Mechanism of a High-Fidelity and a Low-Fidelity DNA Polymerases”, The 9th Symposium on Recent Advances in Biophysics, Taipei, May 26-28, 2004. (Keynote)

  53. “NMR structures of proteins related to DNA damage and cancer”. Symposium on structures, dynamics, and interactions of biological molecules, National Central University, June 4, 2004.

  54. “NMR studies of proteins related to DNA damage and cancer”. University of Carnegie Mellon, July 29, 2004 (Chien Ho, host).

  55. “Stereochemistry Goes a Long Way in Phosphatidylinositol-specific Phospholipase C”, Frontiers of Bioorganic and Natural Product Chemistry Symposium, Seattle, August 26-29, 2004. (Also serve as chair of the organizing committee.)

  56. “Structure and Function of FHA Domains in Signal Transduction”. Tsinghua University, September 15, 2004.

  57. “Learning the Transition State Structure of Phosphatidylinositol-specific Phospholipase C from Bioorganic Approaches”, Eighth ISCOC International Symposium, Hong Kong, December 19-22, 2004.

  58. “NMR studies of proteins related to DNA damage and cancer”. Institute of Bioinformatics and Structural Biology, National Tsing Hua University, December 30, 2004. (Ping-Chiang Lyu, host)

  59. “FHA Domains in Signal Transduction:  from Chemistry to Biology", Department of Chemistry, Case Western Reserve University, Cleveland, Ohio, January 20, 2005. (Robert Salomon, host)

  60. “Protein-Phosphoprotein Interactions – A New Frontier in Structural Biology.” National Defense University, Taipei, Taiwan, March 10, 2005.

  61. “Chemical Approach to Biology: A Case for Caution”. Symposium on Chemistry at the Interface of Biology, Salk Fork Resort, May 14, 2005.

  62. “Structure, Function and Specificity of FHA Domains”. Institute of Pasteur, May 23, 2005.

  63. “Protein-Phosphoprotein Interactions – A New Frontier in Structural Biology.” Biophysical Society Meeting, Hsinchu, May 27, 2005. (Plenary lecture)

  64. “When NMR Beats X-ray Crystallography in the Determination of Protein Structures.” Taiwan Magnetic Resonance Society, Taipei, May 28, 2005. (Inauguration lecture)

  65. “Protein-Phosphoprotein Interactions – A New Frontier in Structural Biology.” National Taiwan University (Chemistry), Taipei, Taiwan, June 10, 2005.

  66. “Protein-Phosphoprotein Interactions – A New Frontier in Structural Biology.” NHRI, Taiwan, Sept 02, 2005.

  67. “When NMR Beats X-ray in Solving Protein Structures”. First Asia-Pacific NMR Symposium, Japan, Nov. 10-11, 2005.

  68. “FHA Domains in Signal Transduction:  a Case for Caution in Chemical Biology”. Dept of Chemistry, SUNY at Buffalo, Nov. 30, 2005. (John Richard, host)

  69. “Structural Biology of Cancer-relevant Proteins”. NHRI Cancer Program, Taipei, June 9, 2006.

  70. “Mechanism of action of high and low fidelity DNA polymerases”. Gordon Research Conference on Enzymes, Coenzymes, and Metabolic Pathways, New Hampshire, July 16-21, 2006. (John Richard and Sue Miller, co-chairs)

  71. “Identification of Histone Demethylases from Sacchromyces cerevisiae”. Biophysics Program, Ohio State University, October 4, 2006. (Ralph Bundschuh, host)

  72. “Structural Biology of Cancer Proteins.” Institutional Seminar of National Taiwan Univ. College of Medicine and University Hospital, December 27, 2006. (Host: 張美惠)

  73. “Specificity of Phosphothreonine Recognition by FHA Domains”. International Conference of Phosphorus Chemistry, Xiamen, China, April 15-21, 2007.

  74. “Structure, Function and Specificity of Phosphothreonine-specific FHA Domains”. Dept of Chemistry, UC Davis, May 15, 2007. (Host: Xi Chen)

  75. “FHA Domain Mediated Signaling Related to DNA Damage and Cancer”. Cellular and Molecular Medicine Program, Academia Sinica, June 1, 2007.

  76. “Specificity of Phosphothreonine Recognition by FHA Domains”. XXIII International Conference on Yeast Genetics and Molecular Biology, Melbourne, Australia, July 2-6, 2007.

  77. “FHA Domain - A Novel Phosphate Counting Switch for Sequential Activation of a Checkpoint Kinase Cascade”, Eli Lilly, Indianapolis, October 4, 2007. (Host: Chuan Shih)

  78. “A phospho-counting switch for sequential activation of a checkpoint kinase cascade”. 2nd International Symposium on Bio-Inspired Engineering (ISBIE), October 8-10, 2007 at the Le Meridien Hotel, Dead Sea, Israel. (www.isbie.org)

  79. “A phospho-counting switch for sequential activation of a checkpoint kinase cascade”. The 2nd Asian-Pacific NMR Symposium, Oct. 12-14, 2007, Hsin-chu, Taiwan (Plenary Lecturer)

  80. “Nuclear Protein NP as a Target for Inhibition of Viral Replication”. International Symposium on Flu Virus, Oct 22, 2007, NHRI/NTUH, Taiwan.

  81. “A phospho-counting switch for sequential activation of a checkpoint kinase cascade”. International Symposium on Proteins: from Chemistry to Biology, October 24-26, 2007, Institute of Biological Chemistry, Academia Sinica, Taiwan.

  82. “FHA Domain in Signal Transductions:  from Chemistry to Biology”. National Tsinghua University Dept of Chemistry, November 21, 2007.

  83. “Oligomerization of the Nucleocapsid Protein as a Target for the Flu Virus”. UC Davis – Academia Sinica Bilateral Conference, December 6-7, 2007, Taipei.

  84. “FHA Domain in Signal Transductions:  from Chemistry to Biology”. Kaoshiung Medical Univdrsity. March 19, 2008.

  85. “From the End to the Beginning”. Great Lakes Regional ACS Meeting, Columbus, Ohio, June 14, 2008.

  86. “A Phospho-counting Switch for Sequential Activation of a Checkpoint Kinase Cascade“. Toulouse University, France, July 7, 2008. (Host: Bernard Salles)

  87. "Nucleocapsid Protein NP as a Target for Inhibition of Flu Virus Replication". World Summit of Antivirals 2008, Kunming, China, July 20-22, 2008.

  88. “FHA Domain in Signal Transduction: Chemistry, Structure, and Biology”. Institute of Chemistry, Academia Sinica, Taipei, Sept 25, 2008. (Host: Sunney Chan)

  89. “A Phospho-counting Switch for Sequential Activation of a Checkpoint Kinase Cascade in S. cerevisiae”. Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Oct 1, 2008. (Host: Sunny Lo)

  90. “FHA Domain in Signal Transduction: Chemistry, Structure, and Biology”. National Cheng Kung University, Tainan, October 2, 2008.

  91. “Structures of FHA domain complexes with phosphoprotein and phosphopeptides with single and multiple pThr sites”. CSMRS-2, Hang-Chou, China, Oct 23-27, 2008.

  92. “Structure, Function and Specificity of Phosphothreonine-specific FHA Domains”. Dept of Biochemistry, UC Riverside, Nov. 4, 2008. (Host: John Shyy)

  93. “Structure, Function and Specificity of FHA Domains in DNA Damage Response Signaling.” Department of Medicinal Chemistry, Purdue University, November 7, 2008.

  94. “Structure and mechanism of a mutagenic DNA polymerase from African Swine Fever Virus”. 4th International Symposium on Biocatalysis and Biotechnology, Academia Sinica, Taipei, Nov 19-21, 2008.

  95. “Structure, Function and Specificity of Phosphothreonine-specific FHA Domains”. National Yangming University, November 24, 2008. (Host: 魏耀揮)

  96. “Cancer Research from the Angle of Structural Biology”. “姆山生物醫學講座”, Taipei Medical University, Nov. 27, 2008.

  97. “Counting Phosphates by FHA Domains”. Taiwan – Japan Proteomics Symposium 2008. Academia Sinica, December 3, 2008.

  98. “FHA, a signaling domain with diverse specificities”. The 6th Asian Biophysical Association (ABA) Symposium, Hong Kong University of Science and Technology, Hong Kong, 11-14 January 2009.

  99. “Molecular Interactions of Biophosphates – from Catalysis to Signaling”. 24th Joint Annual Conference of Biomedical Sciences, Taipei, Taiwan, March 21-22, 2009. (Special Lecturer)

  100. “Protein-phosphoprotein interactions of phosphothreonine-specific FHA domains”. Symposium of Nuclear Magnetic Resonance for Biological Macromolecules 2009 (SNBM2009), Hefei, China, June 25-29, 2009.

  101. “Structure and Mechanism of Mutagenic DNA Polymerase X”. 3rd Asian-Pacific NMR Conference, Korea, Oct 25-28, 2009.

  102. “Protein-phosphoprotein interactions of phosphothreonine-specific FHA domains”. Tsinghua University, Beijing, Nov. 25, 2009. (Host: Yigong Shi)

  103. “Structure and Mechanism of Mutagenic DNA Polymerase X”. Beijing Conference and Exhibition on Instrumental Analysis (
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