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Charcot-Leyden Crystal Protein Staining on Stool Samples 12/11/06 mkm


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Charcot-Leyden Crystal Protein Staining on Stool Samples

12/11/06 MKM





  1. Fix 20 ug of stool in 500 ul of PVA for 20 min.

  2. Use 20 ul of fixed stool on slide for smear.

  3. Dry overnite

  4. 70% EtOH + iodine (until tea colored) 5-10 min. Removes mercuric chloride

(from PVA fix) & hydration

  1. 70 % EtOH 5 min. Removal of iodine & hydra.

  2. 70% EtOH 3 min. Wash & hydration

  3. Trichrome (Wheatley’s) stain 10 min Staining

  4. 90% Alcohol Acidified dip 1-3 sec Destaining

(99.5 ml 90% EtOH + 0.5 ml glacial acetic acid)

  1. Abs EtOH dip x 3 Stops staining

  2. Abs EtOH 3 min. Dehydration

  3. Abs EtOH 3 min. Dehydration

  4. Xylene 5-10 min. Removal of Alcohol

  5. Xylene 5-10 min. Removal of Alcohol

  6. Mount and examine under oil.


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